多位点序列分型应用于致病性黄疸出血群钩端螺旋体分析

发布时间：2018-04-24 01:17

本文选题：钩端螺旋体 + 黄疸出血群　；参考：《中国人兽共患病学报》2015年05期

【摘要】：目的初步探讨MLST(multilocus sequence typing)分型技术在致病性钩端螺旋体中的应用。方法对我国3个省份的39株致病性钩端螺旋体黄疸出血群菌株提取DNA,采用7个位点进行PCR扩增、序列测定,应用eBURST和BioNumerics(Version5.10)软件进行分析。结果 39株菌株呈现5个ST型别,ST1为中国三省份中主要的基因型,占53.85%(21/39)。eBURST分析显示:39株菌株分为2个Clonal complexes和1个singleton,其中Group1占66.67%(26/39),Group2占20.51%(8/39),一个singleton占12.82%(5/39);BioNumerics软件聚类分析显示:39株菌株分为3个Group,与eBURST分析中的分群结构一致。MLST基因型别具有明显的地域性特征。结论 MLST方法可初步应用于致病性钩端螺旋体分子流行病学、种群结构、亲缘进化关系等研究。
[Abstract]:Objective to explore the application of MLST(multilocus sequence typing technique in pathogenic leptospirosis. Methods 39 strains of pathogenic leptospirosis haemorrhage group were extracted from 39 strains from three provinces of China. PCR amplification was performed at 7 loci and sequence was sequenced. EBURST and BioNumericsm Version5.10 software were used to analyze the results. Results 39 strains showed 5 ST-type ST1 genotypes, which were the main genotypes in three provinces of China. The results of 53.85%(21/39).eBURST analysis showed that 39 strains were divided into 2 Clonal complexes and 1 singleton, of which Group1 accounted for 66. 67%, Group1 accounted for 26 / 39%, accounted for 20.51%, and one singleton accounted for 12. 82%. BioNumerics cluster analysis showed that 9 strains were divided into 3 groups, which were consistent with the cluster structure in eBURST analysis. MLST genotypes had obvious regional characteristics. Conclusion MLST method can be applied to the study of molecular epidemiology, population structure and phylogenetic relationship of pathogenic leptospira.
【作者单位】：中国疾病预防控制中心传染病预防控制所;江西省疾病预防控制中心;
【基金】：国家科技重大专项(2012ZX10004-215,2013ZX10004-101) 国家自然科学基金(81471968)联合资助~~
【分类号】：R514.4

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