CHC患者血清TNF-α、IL-10水平及其与病毒载量、ALT、AST关系探讨

发布时间：2018-04-27 17:57

本文选题：慢性丙型肝炎 + 肿瘤坏死因子-α　；参考：《新疆医科大学》2013年硕士论文

【摘要】：目的：研究慢性丙型肝炎患者血清TNF-α、IL-10水平及其与HCV RNA载量、ALT、AST水平的关系,并探讨其导致肝脏损害的机制。方法：用酶联免疫吸附法(ELISA)检测112例慢性丙型肝炎患者、40例健康对照者血清中TNF-α、IL-10的水平。用PCR-荧光探针法检测血清HCVRNA水平。使用全自动生化分析仪检测ALT、AST含量。结果：慢性丙型肝炎患者组TNF-α、IL-10的水平均高于健康对照组(P=0.000,=0.000),在慢丙肝患者中,轻度组TNF-α,的水平均低于中度重度组,而轻度与中度组间差异无统计学意义(P=0.023,P=0.000,P=0.106),而IL-10水平在轻、中、重三组之间差异均无统计学意义(F=1.555,P=0.221)。随着HCV RNA载量的升高,血清IL-10水平逐渐升高,各组之间差异有统计学意义(F=13.429,P=0.000)。而TNF-α在不同HCV RNA载量组之间均无显著性差异(F=1.212,P=0.302)。TNF-α与ALT、AST呈正相关,而与HCV RNA无相关性(P=O.000,P=0.000,P=0.105),IL-10与HCV RNA呈正关,与ALT、AST无相关性(P=0.000,P=0.147,P=0.102), HCV RNA载量与ALT. AST水平无相关性(P=0.065,P=0.071)。结论：血清TNF-a水平随着病情程度的加重升高,IL-10随着HCV RNA载量升高而升高,TNF-α与IL-10是引起丙型肝炎肝脏损害及慢性化的重要因素,检测TNF-α与IL-10水平可作为判断慢性HCV感染情况的指标。
[Abstract]:Objective: to study the relationship between serum TNF- 伪 -IL-10 level and HCV RNA load and alt level in patients with chronic hepatitis C, and to explore the mechanism of liver damage. Methods: the serum levels of TNF- 伪 and IL-10 in 112 patients with chronic hepatitis C and 40 healthy controls were detected by enzyme linked immunosorbent assay (Elisa). The serum HCVRNA level was detected by PCR- fluorescence probe method. The content of alt was measured by automatic biochemical analyzer. Results: the levels of TNF- 伪 IL-10 in patients with chronic hepatitis C were higher than those in healthy controls. In patients with chronic hepatitis C, the levels of TNF- 伪 in mild group were lower than those in moderate group, but there was no significant difference between mild group and moderate group. There was no significant difference among the three groups. With the increase of HCV RNA load, the serum IL-10 level increased gradually, and the difference between the groups was statistically significant. However, there was no significant difference in TNF- 伪 between different HCV RNA load groups. There was a positive correlation between TNF- 伪 and alt AST, but no correlation between TNF- 伪 and HCV RNA. There was no positive correlation between IL-10 and HCV RNA, and there was no correlation between TNF- 伪 and ALTAST, and there was no correlation between HCV RNA load and ALT. The level of AST was not correlated with 0.065 and 0.071. Conclusion: the level of serum TNF-a increases with the severity of the disease. IL-10 increases with the increase of HCV RNA load. TNF- 伪 and IL-10 are important factors causing liver damage and chronic hepatitis C. Detection of TNF- 伪 and IL-10 levels can be used as an index to judge chronic HCV infection.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.63

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