血清GPC3、GS和HSP70检测在乙肝相关肝癌诊断中的应用

发布时间：2018-04-29 08:24

本文选题：磷脂酰肌醇聚糖 + 热休克蛋白　；参考：《广东医学》2015年06期

【摘要】：目的探讨磷脂酰肌醇聚糖3(glypican3,GPC3)、热休克蛋白70(heat shock protein 70,HSP70)、谷氨酰胺合成酶(glutamine synthetase,GS)在血清中的浓度及对乙肝相关肝癌(HCC)的诊断价值。方法收集100例乙肝相关HCC患者(HCC组)、20例乙肝肝硬化者(肝硬化组)、20例慢性乙肝患者(慢性乙肝组)及20例正常健康人(正常对照组)血清,用ELISA方法检测GPC3、GS、HSP70的水平。结果 HCC组血清GPC3水平与其余3组差异有统计学意义,GPC3诊断乙肝相关肝癌ROC曲线示AUC为0.697,敏感度84.21%,特异度55.1%。HCC组与肝硬化组及慢乙肝组间血清GS水平差异无统计学意义,GS诊断乙肝相关肝癌ROC曲线示AUC为0.603,敏感度46.67%,而特异度79.66%;HCC组与其余3组血清HSP70水平差异有统计学意义,HSP70诊断乙肝相关肝癌ROC曲线示AUC为0.725,敏感度58.06%,特异度89.55%;针对AFP200 ng/m L的HCC患者,仅HSP70的血清检测AUC达0.725,其他指标对于小肝癌的诊断AUC均未达0.7。结论 ELISA法检测血清GPC3、GS、HSP70水平对诊断乙肝相关肝癌无临床实际价值。
[Abstract]:Objective to investigate the serum concentration of phosphatidylinositol 3 glypican3 (GPC 3), heat shock protein (70(heat shock protein) 70 HSP70, glutamine synthetase (GST) and its diagnostic value in hepatitis B related hepatocellular carcinoma (HCC). Methods the serum samples of 20 patients with hepatitis B cirrhosis (20 patients with chronic hepatitis B) and 20 healthy people (normal control group) were collected from 100 patients with HCC. The levels of GPC3 and GSS-HSP70 were detected by ELISA method. Results the serum GPC3 level in HCC group was significantly higher than that in the other three groups. The ROC curve of GPC3 in the diagnosis of Hepatitis B related liver cancer showed that AUC was 0.697, sensitivity was 84.21. There was no significant difference in serum GS level between 55.1%.HCC group, cirrhosis group and chronic hepatitis B group. The ROC curve showed that AUC was 0.603 and sensitivity was 46.67.The serum HSP70 level of 79.66HCC group was significantly different from that of the other three groups. The ROC curve of HSP70 showed AUC was 0.725, sensitivity 58.06 and specificity 89.55; For HCC patients with AFP200 ng/m L, Only the serum AUC of HSP70 was 0.725, and AUC of other indexes was not 0.725 for small hepatocellular carcinoma. Conclusion determination of serum GPC3 GSH SP70 by ELISA is of no clinical value in the diagnosis of hepatitis B associated hepatocellular carcinoma.
【作者单位】：中山大学附属第三医院感染科;海南医学院附属医院感染科;中山大学肿瘤防治中心、华南肿瘤学国家重点实验室肝胆科;
【基金】：科技重大专项“十二五”课题(编号:2012ZX10004-902)
【分类号】：R735.7;R512.62

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