云南临沧HIV阳性者血液中弓形虫SAGSAG2基因位点分析

发布时间：2018-04-29 15:59

  本文选题：弓形虫 + HIV阳性　； 参考：《中国血吸虫病防治杂志》2015年01期

【摘要】：目的初步了解云南临沧HIV阳性者血液中弓形虫的基因型特征。方法运用巢式PCR法对弓形虫SAG2基因的两个片段分别进行基因提取、扩增,然后对PCR产物进行回收纯化、酶切及测序,并与弓形虫基因I型标准株进行对比鉴定。结果从170份HIV阳性者全血样本中分别扩增出35个弓形虫SAG2基因(241、221 bp),从其中扩增阳性的全血样本中各选择4份分别进行酶切,扩增出目的基因片段,进一步从酶切样本中选择2份与弓形虫基因标准株RH株进行对比,酶切SAG2基因(241 bp)显示血液样本基因型为Ⅰ型或Ⅱ型,酶切SAG2基因(221 bp)确定基因型均为Ⅰ型。结论初步确定云南临沧HIV阳性者弓形虫基因型以Ⅰ型为主。
[Abstract]:Objective to investigate the genotypic characteristics of Toxoplasma gondii in the blood of HIV positive patients in Lincang, Yunnan Province. Methods two fragments of SAG2 gene of Toxoplasma gondii were extracted and amplified by nested PCR method. The PCR products were purified, digested and sequenced, and compared with the standard strain of Toxoplasma gondii gene type I. Results 35 samples of Toxoplasma gondii SAG2 gene were amplified from 170 whole blood samples with positive HIV. Four samples of Toxoplasma gondii SAG2 gene were digested by enzyme digestion, and the target gene fragments were amplified. Furthermore, two strains of Toxoplasma gondii gene standard strain RH were selected from the digested samples. The results showed that the genotypes of blood samples were type 鈪，

本文编号：1820612