基质辅助激光解吸电离飞行时间质谱技术筛选乙型肝炎肝硬化至肝癌过程中血清标志蛋白的初步研究

发布时间：2018-04-30 20:31

本文选题：肝肿瘤 + 肝硬化　；参考：《中国全科医学》2017年20期

【摘要】：目的应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术分析乙型肝炎肝硬化至肝癌过程中血清标志蛋白的变化,以期发现早期肝癌甚至癌前病变时的血清标志物。方法选取2010—2012年首都医科大学附属北京世纪坛医院收治的乙型肝炎肝硬化患者240例,对其进行随访,为期3年,每6个月抽血1次,保存血清。其中12例患者在随访过程中确诊为原发性肝癌,2例患者留取血样量不足,仅对10例患者的血样进行分析。对完成随访未进展为肝癌的209例乙型肝炎肝硬化患者按照性别、年龄匹配选取40例。并选取同期本院体检中心健康者的血样40例。应用MALDI-TOF-MS技术分析患者血清蛋白质组学的变化。结果应用CM10芯片共检测出109个蛋白峰,其中具有差异的蛋白质分子量分别为:4 350.8 Da、6 858.5 Da、7 571 Da、15 125 Da、15 893 Da。其中4 350.8 Da随着肝癌的发生逐渐呈上调趋势,6 858.5 Da、7 571 Da、15 125 Da、15 893 Da随着肝癌的发生呈下调趋势。结论随着肝癌的发生,分子量为4 350.8 Da的蛋白质呈逐渐上调趋势,其余6 858.5 Da、7 571 Da、15 125 Da、15 893 Da蛋白质呈逐渐下调趋势。
[Abstract]:Objective to analyze the changes of serum marker proteins in the process of hepatitis B cirrhosis and liver cancer by matrix assisted laser desorption and ionization time of flight mass spectrometry (MALDI-TOF-MSM), in order to find the serum markers of early liver cancer and even precancerous lesions. Methods 240 patients with hepatitis B cirrhosis treated in Beijing Century altar Hospital affiliated to Capital Medical University from 2010 to 2012 were followed up for 3 years. Among them, 12 cases were diagnosed as primary liver cancer during follow-up, 2 cases had insufficient blood sample, only 10 cases were analyzed. Forty patients with hepatitis B cirrhosis were selected according to their sex and age. Blood samples were collected from 40 healthy subjects in the same period. The changes of serum proteomics were analyzed by MALDI-TOF-MS technique. Results A total of 109 protein peaks were detected by using CM10 microarray. The molecular weights of the proteins with different molecular weights were respectively 1: 4 350.8 DaC6 858.5 Da571 Da571 Da15125 Da1 15893 Da. Among them, 4 350.8 Da increased gradually with the occurrence of HCC. 6 858.5 Da571 Da125125 Da593 Da showed a downward trend with the occurrence of HCC. Conclusion with the development of HCC, the protein with molecular weight of 4 350.8 Da was gradually up-regulated, while the other 6 858.5 Da571 Da571 Da125125 Da1 15893 Da protein was gradually down-regulated.
【作者单位】：首都医科大学附属北京世纪坛医院消化内科;
【分类号】：R512.62;R575.2;R735.7

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