不同体检人群中乙肝病毒大蛋白的检测意义的探索

发布时间：2018-05-01 02:24

  本文选题：乙型肝炎病毒 + 乙肝病毒大蛋白　； 参考：《浙江大学》2014年硕士论文

【摘要】：1背景 世界上有20亿人感染过HBV,目前全世界慢性HBV感染患者至少有3.8亿,其中慢性HBV携带者75%分布在东南亚和次撒哈拉地区,而我国就占了1.5亿,其中有200万人为慢性乙肝患者,每年因此病死亡的人已近50万。估计全球每年有108-200万人直接死于HBV持续感染。我国是乙肝的高流行地区,乙肝病毒感染是极为重要的公共卫生问题。而在学生升学体检、公务员体检、征兵体检以及餐饮等行业中都对乙肝病毒的检测有一定的规定。因此体检中如何真实、快速的反映体检人员的乙肝情况成为急待解决的问题,目前主要采取检测HBV-DNA载量的方法来评估HBeAg阴性患者体内的乙肝病毒复制情况,但此方法操作繁琐、成本投入大、实验要求高等原因,难以推广应用于大批量体检标本的检测。 2目的 通过检测公务员、学生、士兵等体检人员中乙肝病毒大蛋白(HBV-LP),乙肝病毒DNA(HBV DNA)以及乙肝两对半(HBV-M)的情况,探讨乙肝病毒大蛋白用于公务员、学生、士兵、餐饮人员等不同体检人员中的意义,为乙肝的公共体检领域提供新的检测方法。 3方法 乙肝病毒大蛋白检测采用酶联免疫法,试剂由北京热景生物技术公司提供,cut-off值为0.105；HBV-M血清标志物检测采用酶联免疫法,试剂盒购自上海科华生物有限公司；HBV-DNA定量检测采用实时荧光定量RT-PCR,试剂盒购自广州达安生物公司,以HBV DNA拷贝数≥1×103/ml作为HBV-DNA阳性标准；谷丙转氨酶检测采用化学法,试剂盒购自上海申能生物有限公司,以上均严格按试剂说明书进行操作,有效期内使用。采用SPSS12.0软件进行统计学分析.计量资料用X±S表示。HBV-LP与HBV-DNA阳性率的比较采用χ2检验,p0.05判定有统计学意义；HBV-LP S/OD值与HBV-DNA拷贝数之间的相关性采用线性相关分析。 4结果 4.1HBV-LP法与HBV-DNA法检测709例中HBsAg阳性的体检人员的检测结果差异无统计学意义,χ2值和P值分别为1.47和0.23。 4.2不同乙肝两对半模式中HBV-LP法与HBV-DNA法的检测结果差异均无统计学意义,χ2值和P值分别为2.67,0.60,0.00和0.10,0.44,1.00。 4.3HBV-LP S/OD值与HBV-DNA的拷贝数呈正相关性(r=0.959,P0.05)。 5结论乙型肝炎病毒大蛋白(HBV-LP)在能够有效、真实地反映HBV病毒复制情况,该方法可以在公务员、学生、士兵等体检中进行推广
[Abstract]:1 background There are 2 billion people in the world who have been infected with HBV. At present, there are at least 380 million patients with chronic HBV infection in the world. Among them, 75% of the chronic HBV carriers are in Southeast Asia and Sub-Saharan region, and China accounts for 150 million, of which 2 million are chronic hepatitis B patients. Nearly 500000 people die each year from the disease. An estimated 108 to 2 million people worldwide die of persistent HBV infection every year. Hepatitis B virus infection is an extremely important public health problem in China. However, there are certain regulations on HBV detection in students' entrance examination, civil servant physical examination, conscription medical examination and catering industry. Therefore, it is urgent to solve the problem of how to truly and quickly reflect the hepatitis B situation of people in physical examination. At present, the method of detecting HBV-DNA load is mainly used to evaluate HBV replication in HBeAg negative patients, but the operation of this method is cumbersome. Because of the high cost and high demand, it is difficult to be applied to the detection of large numbers of physical examination specimens. 2 purpose In order to explore the application of hepatitis B virus large protein in civil servants, students, soldiers and so on, the hepatitis B virus large protein (HBV-LPU), hepatitis B virus DNA(HBV DNA (HBV DNA(HBV DNA) and hepatitis B virus (HBV-MN) were used in civil servants, students, soldiers, etc. The significance of different physical examination personnel, such as catering personnel, provides a new method for the detection of hepatitis B in the field of public health examination. 3 method The detection of hepatitis B virus large protein was performed by enzyme linked immunosorbent assay (Elisa), and the serum marker of HBV-M was determined by enzyme linked immunosorbent assay (Elisa), which was provided by Beijing Hejing Biotechnology Company with a cut-off value of 0.105 渭 m. The kit was purchased from Shanghai Kehua Biological Co., Ltd. The quantitative detection of HBV-DNA was carried out by real-time fluorescence quantitative RT-PCR.The kit was purchased from Guangzhou Da'an Biological Company, with the copy number of HBV DNA 鈮，

本文编号：1827323