2011-2014年威海市HIV抗体筛查阳性标本的确证结果分析

发布时间：2018-05-04 03:21

本文选题：艾滋病病毒 + 筛查试验　；参考：《中国艾滋病性病》2015年11期

【摘要】：目的对威海市2011-2014年艾滋病病毒(HIV)抗体筛查阳性标本的确证试验结果进行分析,为威海艾滋病防治工作提供技术支持。方法 2011-2014年威海市辖区内25家筛查实验室,采用第四代酶联免疫吸附试验(ELISA)及胶体硒试验,对送检的及本筛查中心实验室检出的220例筛查阳性标本,使用蛋白印迹试验(WB)进行确证试验。结果 220例筛查阳性标本中,确证HIV-1抗体阳性185例,阳性率为84.09%;确证阴性11例,阴性率为5.00%;HIV抗体不确定24例,占10.90%。筛查阳性标本来源于医疗、疾病预防控制中心、采供血机构,其中采供血机构的阳性率为63.64%(14/22)。确证的185例中,条带gp160和gp120出现率为100%,p55、p39条带的阳性率分别为62.70%(116例)、61.08%(113例)。在随访到的不确定标本中,条带含有gp160、p24的带型转阳率很高。二次确证有13.92%(11/79)的标本和一次确证的带型不一致。结论 HIV抗体初筛试验结果存在一定的假阳性,HIV抗体结果以WB结果为准;WB试验中不确定标本需做好随访工作,尽量减少潜在新发感染者的流失。一次、二次确证不一致的标本可作为研究新发感染的依据之一。
[Abstract]:Objective to analyze the positive results of HIV antibody screening in Weihai City from 2011 to 2014, and to provide technical support for the prevention and treatment of HIV / AIDS in Weihai. Methods Twenty-five screening laboratories in Weihai City from 2011 to 2014 were tested with ELISA4 and colloidal selenium test. 220 positive samples were detected in the laboratory of our screening center. Western blotting test (WB) was used for confirmation test. Results the positive rate of HIV-1 antibody was 84.09 and the negative rate was 5.000.The positive rate was 10.90% and the negative rate was 5.000.The positive rate was 84.09, and the negative rate was 5.000.The positive rate of HIV-1 antibody was 10.90%, while the negative rate was 5.000.The positive rate was 10.90%. The positive samples were obtained from medical treatment, CDC, and blood collection organization. The positive rate of blood collecting and supplying organizations was 63.64 / 14 / 22. Of the 185 confirmed cases, the positive rates of gp160 and gp120 were 100% and 62.70%, respectively. The positive rates of p55 and p39 bands were 62.70% and 61.08%, respectively. The positive rate of band type with gp160 and p24 was high in the follow-up samples. There were 13.92% and 13.92% 11 / 79) samples and one confirmed band pattern was not consistent. Conclusion there are some false positive HIV antibody results in the primary screening test of HIV antibody. It is necessary to do a good follow-up work to reduce the loss of potential newly infected persons by using WB results. First, second confirmation of inconsistent specimens can be used as a basis for the study of new infections.
【作者单位】：威海市疾病预防控制中心;
【分类号】：R512.91

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