Ⅱ型弓形虫和血吸虫重叠感染对小鼠肝纤维化作用的实验研究

发布时间：2018-05-04 03:43

本文选题：Ⅱ型弓形虫 + 日本血吸虫　；参考：《安徽医科大学学报》2016年02期

【摘要】：目的观察Ⅱ型弓形虫和日本血吸虫重叠感染对小鼠肝纤维化的影响,探讨弓形虫感染诱导的抗感染免疫对血吸虫感染致小鼠肝脏纤维化的免疫调节作用。方法BALB/c小鼠建立重叠感染模型:预先感染血吸虫(模型Ⅰ组);预先感染PRU株弓形虫(模型Ⅱ组);对照:只感染尾蚴组和正常对照组。取上述小鼠血液及肝脏,HE和Masson胶原纤维染色法观察肝脏虫卵肉芽肿大小和肝纤维化面积;检测纤维化指标透明质酸(HA)和肝脏羟脯氨酸(HYP)含量。结果模型Ⅰ组小鼠肝脏病理及纤维化指标检测与相应对照组未见显著差异;模型Ⅱ组动物血清HA和肝脏HYP显著低于相应对照组(P0.001),肝脏虫卵肉芽肿大小和纤维化面积均显著减小(P0.001)。结论预先感染Ⅱ型弓形虫可能诱导机体的Th1型应答的偏移,在此微环境下可负向调控血吸虫肝脏纤维化状态的Th2应答,从而延缓和减弱肝纤维化的发生和发展。
[Abstract]:Objective To observe the effect of the overlapping infection of Toxoplasma gondii and Schistosoma japonicum on liver fibrosis in mice, and to explore the immunomodulatory effect of anti infection immunity induced by Toxoplasma infection on liver fibrosis induced by Schistosoma infection in mice. Methods BALB/c mice were established with an overlapping infection model: pre infection of Schistosoma (model I) and pre infection of PRU pantograph. In the control group, the blood and liver of the mice were infected only with cercariae and normal controls. The size of liver egg granuloma and the area of liver fibrosis were observed by HE and Masson collagen staining, and the content of hyaluronic acid (HA) and liver hydroxyprolyl (HYP) in the liver were detected. In the model II group, the serum HA and the liver HYP were significantly lower than that in the corresponding control group (P0.001), and the size and the fibrosis area of the liver egg granuloma were significantly decreased (P0.001). Conclusion the pre infection type II Toxoplasma gondii may induce the migration of Th1 type response in the body, which can be negative in this microenvironment. Regulation of Th2 response to hepatic fibrosis in schistosomiasis can delay and attenuate the occurrence and development of liver fibrosis.

【作者单位】：安徽医科大学病原生物学教研室;安徽省庐江县人民医院检验科;安徽医科大学第一附属医院检验科;
【基金】：国家自然科学基金(编号:81471983;81171606) 安徽高校省级自然科学研究项目(编号:KJ2014A106)
【分类号】：R532.21;R531.8

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