乙肝病毒相关肝细胞肝癌与IL-32表达相关性研究

发布时间：2018-05-04 06:24

本文选题：肝细胞癌 + 乙型肝炎病毒　；参考：《中华肿瘤防治杂志》2017年12期

【摘要】：目的肝细胞肝癌(hepatocellular carcinoma,HCC)是最常见的恶性肿瘤之一,白细胞介素(interleukin,IL)-32是新发现的一种细胞因子,在HCC中的研究较少。本研究探讨其在乙肝病毒(heptitis B virus,HBV)相关HCC患者外周血、肝组织以及体外培养的HepG2、HepG2 2.15细胞株中表达水平的变化。方法选择2013-01-01-2016-06-30在南通市第三人民医院住院的HBV相关肝硬化(liver cirrhosis,LC)27例和HBV-HCC 29例作为研究对象,选择体检健康者17名作为对照组,采用酶联免疫吸附方法检测外周血IL-32。选择外科手术切除HBV-HCC、癌旁LC标本20份,采用免疫组织化学的方法检测肝组织中IL-32表达情况。对HepG2、HepG2 2.15细胞进行培养,72h后采集上清液,采用蛋白印迹法检测IL-32。结果 HBV-HCC患者外周血IL-32表达水平为(479.46±204.46)ng/L,高于HBV-LC患者(333.61±162.76)ng/L;HBV-LC患者高于健康对照组为(75.58±9.90)ng/L,P0.005;HBV-HCC癌组织中IL-32表达水平高于癌旁LC组织,Z=2.725,P=0.006;HepG2细胞株与HepG2.2.15细胞株培养72h后,上清液中未能检测到IL-32。结论 IL-32在HBV相关HCC外周血、HCC癌组织中均处于过度表达状态,在HBV-HCC的发生发展过程中起到一定的作用。
[Abstract]:Objective Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Interleukin-interleukin-32 (IL-32) is a newly discovered cytokine, which is less studied in HCC. The aim of this study was to investigate the expression of HepG2 2. 15 cells in peripheral blood, liver tissue and HepG2 2. 15 cell line of hepatitis B virus heptitis B virus (HCC) related patients. Methods 27 patients with HBV associated cirrhosis and 29 patients with HBV-HCC were enrolled in the study in Nantong third people's Hospital from January to June 2016-30. 17 healthy subjects were selected as control group. Peripheral blood IL-32 was detected by enzyme-linked immunosorbent assay (Elisa). The expression of IL-32 in liver tissue was detected by immunohistochemical method in 20 specimens of adjacent LC after surgical resection of HBV-HCC. HepG2 2. 15 cells were cultured for 72 h and then supernatant was collected. IL-32 was detected by Western blot. Results the level of IL-32 expression in peripheral blood of HBV-HCC patients was 479.46 卤204.46 ng / L, which was higher than that of HBV-LC patients (333.61 卤162.76 ng / L) and healthy controls (75.58 卤9.90 ng / L P 0.005). The level of IL-32 expression was higher than that of adjacent LC tissues. After 72 hours of culture, IL-322was not detected in the supernatant. Conclusion IL-32 is overexpressed in peripheral blood of HBV related HCC and plays an important role in the development of HBV-HCC.
【作者单位】：南通市第三人民医院感染科;南通市第三人民医院病理科;
【基金】：南通市社会发展项目(HS20120019)
【分类号】：R512.62;R735.7

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