无锡市腹泻病人沙门菌的病原学特征及分子分型研究

发布时间：2018-05-04 17:01

本文选题：沙门菌 + 耐药性　；参考：《中国人兽共患病学报》2017年04期

【摘要】：目的分析无锡市腹泻人群中分离的沙门菌的流行特征;同时比较主要血清型菌株间的脉冲场凝胶电泳(PFGE)带型差异,为沙门菌感染的防控提供实验数据。方法收集2015年无锡市腹泻病人的粪便标本,分别进行沙门菌的分离鉴定、药敏试验、血清型分型以及PFGE分型分析。结果 756份粪便标本中共分离到32株沙门菌,阳性率为4.23%;检出时间主要集中在5~10月份,感染人群以老年人为主。药敏试验说明无锡地区的沙门菌对氨苄西林的耐药率最高,达56.25%;对环丙沙星和头孢他啶的耐药率最低,均为6.25%。32株沙门菌共鉴定出11种血清型,以肠炎沙门菌和鼠伤寒沙门菌为主,分别占31.25%和21.88%。对这2种血清型的沙门菌进行PFGE分析,显示肠炎沙门菌所有带型的相似度均在85%以上,鼠伤寒沙门菌的带型各不相同。结论无锡市沙门菌的感染具有明显季节和年龄分布差异性,流行的优势血清型为肠炎沙门菌。无锡市需同时加强对食品和环境中沙门菌的监测。
[Abstract]:Objective to analyze the epidemic characteristics of Salmonella isolated from diarrhea population in Wuxi and to compare the difference of PFGE bands among the main serotype strains in order to provide experimental data for the prevention and control of Salmonella infection. Methods fecal specimens from diarrhea patients in Wuxi City in 2015 were collected and identified by isolation and identification of Salmonella, drug sensitivity test, serotype typing and PFGE typing analysis. Results A total of 32 Salmonella strains were isolated from 756 fecal specimens with a positive rate of 4.23.The detection time was mainly in May and October. The drug sensitivity test showed that the resistant rate of salmonella to ampicillin was the highest in Wuxi area (56.25%), the resistance rate to ciprofloxacin and ceftazidime was the lowest (6.25.32 strains), and 11 serotypes were identified. Salmonella enteritidis and Salmonella typhimurium were dominant, accounting for 31.25% and 21.88%, respectively. The PFGE analysis of the two serotypes showed that the similarity of all bands of Salmonella enteritidis was more than 85%, and the bands of Salmonella typhimurium were different. Conclusion the infection of Salmonella in Wuxi city has obvious seasonal and age distribution differences, and the predominant serotype is Salmonella enteritidis. Wuxi city should also strengthen the monitoring of salmonella in food and environment.
【作者单位】：无锡市疾病预防控制中心;
【基金】：无锡市卫生局科研项目(No.Q201508)资助~~
【分类号】：R446.5;R516.3

【相似文献】