肝泡型包虫病患者血清sPD-1、sPD-L1的表达水平及其临床意义

发布时间：2018-05-06 13:32

本文选题：肝泡型包虫病 + 协同信号分子　；参考：《青海大学》2017年硕士论文

【摘要】：目的:研究肝泡型包虫病(hepatic alveolar echinococcosis,HAE)患者外周血中可溶性程序性死亡蛋白1(soluble programmed death protein1,s PD-1)和可溶性程序性死亡蛋白配体1(soluble programmed death protein1ligand 1,s PD-L1)的表达水平并初步探讨其临床意义。方法:1.研究对象共40例,其中实验组(HAE患者)20例,对照组(健康体检者)20例。收集HAE患者术前血清样本及相应临床病例资料(如年龄、性别、肝功能、血常规等)。2.采用酶联免疫吸附分析法(ELISA)检测上述研究对象外周血上清中s PD-1、s PD-L1的表达水平,并分析s PD-1、s PD-L1与HAE患者各项临床指标的相关性。结果:1.s PD-1的表达水平在实验组和对照组间差异有统计学意义(48.05±35.71pg/ml VS.10.72±4.63pg/ml,t=4.635,P=0.000);s PD-L1的表达水平在实验组和对照组间差异亦有统计学意义(164.75±116.69 pg/ml VS.69.58±48.30pg/ml,t=3.370,P=0.002)。2.s PD-1、s PD-L1在HAE患者外周血中的浓度呈正相关(r=0.881,P0.01)。3.s PD-1、s PD-L1分别与TBIL、DBIL、ALT、AST水平成正相关(P0.05)。4.HAE患者血清中的s PD-1和s PD-L1的表达水平与年龄、白细胞计数、中性粒细胞计数、嗜酸性粒细胞计数、红细胞计数、血红蛋白含量、白蛋白含量及包虫病灶大小均无相关性。结论:在HAE患者及健康体检人体内均可检测到一定量s PD-1、s PD-L1,但在HAE患者的外周血中均异常增高,且s PD-1、s PD-L1的表达水平与TBIL、DBIL、ALT、AST等肝损伤指标存在一定的相关性,提示s PD-1、s PD-L1可能参与了HAE的发生发展,但它们的具体生物学意义还有待进一步研究。
[Abstract]:Objective: to study the expression levels of soluble 1(soluble programmed death protein 1 (PD-1) and soluble programmed death protein (1(soluble programmed death protein1ligand 1) PD-L1) in peripheral blood of patients with hepatic alveolar echinococcus coensis (HAE) and to explore its clinical significance. Method 1: 1. There were 40 subjects, including 20 patients with HAE in the experimental group and 20 patients in the control group. To collect serum samples and clinical data of HAE patients before operation (such as age, sex, liver function, blood routine, etc.). Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of sPD-1 PD-L1 in the supernatant of peripheral blood, and the correlation between the expression of sPD-1 PD-L1 and the clinical indexes of HAE patients was analyzed. Results there was significant difference in the expression of PD-1 between the experimental group and the control group (48.05 卤4.63pg / ml 卤4.63pg / ml). There was also a significant difference between the experimental group and the control group (164.75 卤116.69 pg/ml VS.69.58 卤48.30 pg/ml VS.69.58 卤48.30pg / ml). 2.sPD-1s PD-L1 was positive in the peripheral blood of HAE patients. The expression levels of s PD-1 and s PD-L1 in serum of patients with HAE were positively correlated with the levels of serum s PD-1 and s PD-L1. There was no correlation between leukocyte count, neutrophil count, eosinophil count, erythrocyte count, hemoglobin content, albumin content and the size of hydatid foci. Conclusion: a certain amount of sPD-1P PD-L1 was detected in HAE patients and healthy volunteers, but the expression of sPD-1 PD-L1 was increased in peripheral blood of HAE patients, and the expression level of sPD-1 PD-L1 was correlated with liver injury indexes such as liver injury. These results suggest that sPD-1 PD-L1 may be involved in the genesis and development of HAE, but their biological significance needs further study.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R532.32

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