抗OPN抗体对沙鼠肝多房棘球蚴组织的侵袭性影响的远期观察结果及意义

发布时间：2018-05-08 02:22

本文选题：骨桥蛋白 + 抗体　；参考：《石河子大学》2014年硕士论文

【摘要】：目的：在沙鼠肝多房棘球蚴动物模型基础上，在给予抗OPN抗体干预沙鼠多房棘球蚴后，通过检测MMP-2、TGF-β1因子的表达，探究抗OPN抗体与多房棘球蚴泡囊浸润性生长、转移可能存在的相关性。方法：我们使用多房棘球蚴原头节混悬液(0.15ml/只)，在开腹直视下对沙鼠肝脏穿刺接种，制造肝多房棘球蚴动物模型。实验中取4-6周龄健康雌性长爪沙鼠120只（305）g，随机分为：模型组、实验组、对照组。模型组仅接种多房棘球蚴组，实验组接种多房棘球蚴同时外源性注射抗OPN抗体干预，而对照组在接种多房棘球蚴同时予以注射兔血清（抗OPN抗体溶剂）干预。再将每组肝多房棘球蚴沙鼠随机分成6小组，每小组10只，为感染140d、180d、220d、260d组。于相应时间点处死各组沙鼠，观察多房棘球蚴的生长、转移等情况；免疫组化法测定肝多房棘球蚴组织中MMP-2、TGF-β1因子的表达。结果： 1.140天、180天、220天时，实验组与对照组、实验组与模型组囊湿重有统计学差异（F值分别为8.647，36.96，9.12，p＜0.05），而对照组与模型之间比较无统计学差异（P㧐0.05）。 2.观察泡状棘球蚴转移现象，胸廓淋巴结转移率较高，而肺转移少见，未见脑转移灶。统计结果显示：140天、180天、组时实验组与对照组、实验组与模型组胸廓淋巴结转移率有显著性差异（P㩳0.05），220天组时实验组与对照组，实验组与模型组胸廓淋巴结转移率无显著性差异（P㧐0.05）。 3. HE染色结果显示:在140天、180天、220天时间段，实验组与对照组、实验组与模型组间E.m囊壁病理的改变无显著性差异（P㧐0.05）。 4.免疫组化染色结果显示：肝多房棘球蚴组织中可见MMP-2、TGF-β1因子不同程度的表达，定位于细胞浆，主要分布在肝多房棘球蚴纤维囊壁的生发层，细胞浆中可见黄色、棕黄色或棕褐色等阳性颗粒；MMP-2在实验组、对照组与模型组免疫组化染色阳性细胞率比较差异无统计学意义(P㧐0.05)。而实验组TGF-β1阳性细胞率在在20天、60天、100天、140天、180天、及220天时间段，，TGF-β1在实验组与对照组、实验组与模型组间免疫组化染色阳性细胞率比较无显著性差异(P0.05)，100天组、140天组、180天组时TGF-β1的阳性细胞表率达在实验组与对照组、实验组与模型组比较有显著性差异(P㩳0.05)。结论：MMP-2、TGF-β1因子共同参与了沙鼠肝泡状棘球蚴多房棘球蚴侵袭转移进程进程。通过抗OPN抗体干预可使肝多房棘球蚴组织中TGF-β1因子的表达减低，表明抗OPN抗体干预可能是抑制肝多房棘球蚴中晚期侵袭性生长的机制之一。
[Abstract]:Objective: to investigate the expression of MMP-2 TGF- 尾 1 factor in multilocularis of gerbils by anti OPN antibody, and to explore the infiltrating growth of Echinococcus multilocularis with anti OPN antibody. The possible relevance of the transfer. Methods: a multilocular echinococcus multilocularis model was established by inoculation of gerbil liver with 0.15ml / L Echinococcus multilocularis suspension. 120 healthy female gerbils aged 4-6 weeks were randomly divided into three groups: model group, experimental group and control group. The model group was only inoculated with multi-loculed echinococcus group, the experimental group was inoculated with multi-loculed echinococci and exogenous anti-OPN antibody was injected, while the control group was injected with rabbit serum (anti-OPN antibody solvent) at the same time. Each group was randomly divided into 6 groups, 10 rats in each group. The growth and metastasis of echinococcus multilocularis were observed, and the expression of MMP-2 and TGF- 尾 1 in hepatic hydatid tissues were detected by immunohistochemical method. Results: At 1.140 days after 180 days and 220 days, there was a significant difference in the wet weight of the capsule between the experimental group and the control group, the experimental group and the model group, and the F value was 8.647g / 36.96.96 / 9.12p < 0.05 respectively, but there was no significant difference between the control group and the model group (P < 0.05). 2. The metastatic rate of thoracic lymph nodes was higher than that of lung metastasis. The statistical results showed that there were significant differences between the experimental group and the control group at 140 days to 180 days, and the rates of thoracic lymph node metastasis between the experimental group and the model group at 220 days. There was no significant difference between the experimental group and the model group in the rate of thoracic lymph node metastasis. 3. The results of HE staining showed that there was no significant difference between the experimental group and the control group and between the experimental group and the model group in the pathological changes of the E m cyst wall at the time of 140 days, 180 days and 220 days, and there was no significant difference between the experimental group and the control group. 4. Immunohistochemical staining showed that the expression of MMP-2TGF- 尾 1 was localized in the cytoplasm, mainly in the germinal layer of the fibrous cyst wall of the hepatic hydatid, and yellow in the cytoplasm of the cells, and the expression of MMP-2TGF- 尾 1 was observed in different degree in the liver hydatid tissues, and the expression of MMP-2TGF- 尾 1 was localized in the cytoplasm. There was no significant difference in the positive rate of MMP-2 between the control group and the model group by immunohistochemical staining. The positive rate of TGF- 尾 1 in the experimental group was at 20 days, 60 days, 100 days, 140 days, 180 days, and 220 days, the rates of TGF- 尾 1 positive cells were found in the experimental group and the control group. There was no significant difference in the positive rate of TGF- 尾 1 between the experimental group and the model group. There was no significant difference between the experimental group and the model group. The positive cell rate of TGF- 尾 1 was higher in the experimental group and the control group than in the model group, and there was a significant difference between the experimental group and the model group. Conclusion 1: MMP-2 TGF- 尾 1 is involved in the process of invasion and metastasis of hydatid hydatid in gerbils. The expression of TGF- 尾 1 in hepatic hydatid tissues was decreased by the intervention of anti OPN antibody, which indicated that the intervention of anti OPN antibody might be one of the mechanisms of inhibiting the advanced invasive growth of hepatic hydatid.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R-332;R532.32

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