人感染的布鲁氏菌的检测鉴定与MLVA基因分析的研究

发布时间：2018-05-09 00:16

本文选题：布鲁氏菌 + 布鲁氏菌病　；参考：《新疆医科大学》2013年硕士论文

【摘要】：目的收集新疆地区人感染的布鲁氏菌,对其进行检测、鉴定和MLVA基因分型研究。方法通过主诉、体征、病原接触史等收集疑似布鲁氏菌病患者62例,对疑似布鲁氏菌病患者进行虎红平板试验,进行血液培养以分离细菌,将培养出的细菌进行革兰染色、触酶、氧化酶和Vitck2Compact等鉴定试验确定了12株布鲁氏菌。使用PCR方法对分离的12株布鲁氏菌进行MLVA的16个位点进行检测,结果测序后进行拼接、重复序列分析后进行比对。结果疑似布鲁氏菌病62例有34例表现为虎红平板试验阳性,12株菌通过微生物鉴定方法确定为马耳他布鲁氏菌。使用MLVA-8分型12株菌全部为羊种,其中有7株为45型,1株42型,一株62型。有三株与已知序列均不相同。使用MLVA-11或MLVA-16分型则没有发现与已知序列完全相同的基因型。结论MLVA-16方法可以用于布鲁氏菌的分型,其中Panel2组具有高分辨率的特性。新疆地区人感染的布鲁氏菌以羊种为主,12株菌的MLVA分型结果与已登录序列存在差异,使用MLVA-8分型方法确定三株为新发现基因型,分别表现为(1-5-3-12-2-3-3-2)和(1-5-3-11-2-3-3-2)两种基因型。
[Abstract]:Objective to study the detection, identification and MLVA genotyping of Brucella in Xinjiang. Methods Sixty-two suspected brucellosis patients were collected by main complaint, physical signs and history of exposure to pathogens. The patients with suspected brucellosis were tested with tiger red plate, blood was cultured to isolate bacteria, and the bacteria were stained with Gram. Twelve strains of Brucella were identified by thixozyme, oxidase and Vitck2Compact. PCR method was used to detect 16 MLVA loci of 12 Brucella isolates. The results were sequenced, spliced, repeated sequence analyzed and compared. Results 34 of 62 suspected brucellosis cases were identified as Brucella Malta by microbiological identification. Using MLVA-8, 12 strains were all sheep species, of which 7 strains were type 45, 1 strain 42, and one strain type 62. Three of them were different from the known sequences. No genotypes identical to known sequences were found using MLVA-11 or MLVA-16 typing. Conclusion MLVA-16 method can be used for the typing of Brucella, and the Panel2 group has high resolution characteristic. The results of MLVA genotyping of 12 strains of Brucella in Xinjiang were different from those of the registered strains. Using MLVA-8 typing method, three strains of Brucella were identified as newly discovered genotypes, showing two genotypes: 1-5-3-12-2-3-3-2) and 1-5-3-11-2-3-3-2).
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R516.7

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