云南省急性弛缓性麻痹病例中柯萨奇病毒B5VP1区基因特征分析

发布时间：2018-05-09 01:19

  本文选题：柯萨奇病毒B(CVB) + VP区基因　； 参考：《中国公共卫生》2017年12期

【摘要】：目的分析云南省2015年从急性弛缓性麻痹(AFP)病例中分离到的3株柯萨奇病毒B5(CVB5)VP1区核苷酸基因特征。方法采集RD细胞和L20B细胞对AFP标本进行病毒分离,应用反转录-聚合酶链反应(RT-PCR)扩增病毒VP1基因,目的基因送至基因公司进行序列测定;应用MEGA 6软件对分离到的病毒与基因库代表株进行进化树分析。结果 213例AFP标本中分离到23株非脊髓灰质炎肠道病毒(NPEV),包括3株CVB5;3株CVB5与原型株VP1区之间核苷酸(nt)相似性为80.7%~81.9%,氨基酸(aa)相似性为95.1%~96.5%;系统进化树结果显示,本研究分离的CVB5与山东分离株(JN712675)的nt相似性为91.7%~92%,与安徽分离株(KF751191)的nt相似性为92%~93%。结论云南省2015年从AFP病例中分离到的3株CVB5与山东代表株(JN712675)和安徽代表株(KF751191)相似性较高。
[Abstract]:Objective to analyze the nucleotide characteristics of three coxsackie virus (Coxsackie virus) B5(CVB5)VP1 region isolated from acute flaccid paralysis (AFP) cases in Yunnan Province in 2015. Methods AFP samples were isolated from Rd cells and L20B cells. The virus VP1 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the target gene was sent to the gene company for sequencing. MEGA 6 software was used to analyze the phylogenetic tree of the virus and the representative strains of gene bank. Results Twenty-three strains of non-poliomyelitis enterovirus were isolated from 213 AFP specimens, including 3 strains of CVB5 and 3 strains of CVB5. The similarity of nucleotide sequence was 80.7% and 81.9%, and the similarity of amino acid was 95.1%. The phylogenetic tree results showed that, The NT similarity between CVB5 and Shandong strain JN712675 was 91.7, and that with Anhui isolate KF751191) was 920.The similarity of NT was 92X. Conclusion there is a high similarity between the three CVB5 strains isolated from AFP cases in Yunnan Province in 2015 and the Shandong representative strain JN712675) and the Anhui representative strain KF751191).
【作者单位】： 昆明医科大学公共卫生学院;云南省疾病预防控制中心;
【基金】：国家自然科学基金(81160198)
【分类号】：R440;R512.4
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本文编号：1863937