三种方法检测脑脊液结核分枝杆菌结果分析

发布时间：2018-05-10 11:28

本文选题：夹层杯法 + 脑脊液　；参考：《现代预防医学》2015年05期

【摘要】：目的探讨离心涂片法、夹层杯法和改良罗氏培养法3种方法检测脑脊液中结核分枝杆菌对于结核性脑膜炎的诊断价值。方法对临床确诊的结核性脑膜炎患者、高度可疑结核性脑膜炎患者共50例(结脑组)和30例非结核性脑膜炎患者(对照组)的脑脊液标本采用离心涂片法、夹层杯法和改良罗氏培养法进行检测,对其结果进行对比分析。结果结脑组标本中,离心涂片法、夹层杯法和改良罗氏培养法阳性率分别为1.79%(1/56)、8.93%(5/56)和21.4%(12/56),对照组均未检测到结核菌,比较结脑组3种方法的阳性检测率,其差异有统计学意义(P0.05)。结论培养法阳性率最高,但其检测时间长,不利于对结脑进行及时诊断,夹层杯法较离心涂片法阳性率高,有条件的医院可适当推广。
[Abstract]:Objective to investigate the diagnostic value of mycobacterium tuberculosis in cerebrospinal fluid (CSF) by centrifugal smear method, sandwich cup method and modified Roche culture method. Methods Cerebrospinal fluid (CSF) specimens from 50 patients with tuberculous meningitis, 50 patients with highly suspected tuberculous meningitis (tuberculous meningitis group) and 30 patients with non-tuberculous meningitis (control group) were studied by centrifugal smear. The mezzanine cup method and the modified Roche culture method were used to detect and compare the results. Results the positive rates of centrifuge smear method, sandwich cup method and modified Roche culture method were 1.79% and 8.93%, respectively, and 21.4% of tuberculous bacilli were not detected in the control group. The positive rates of the three methods in the control group were significantly higher than that in the control group (P 0.05). Conclusion the positive rate of culture method is the highest, but the detection time is long, which is not conducive to the timely diagnosis of nodular encephalon. The positive rate of sandwich cup method is higher than that of centrifugal smear method.
【作者单位】：辛集市中医院;石家庄市第五医院;河北省胸科医院;首都医科大学附属北京胸科医院;北京市结核病胸部肿瘤研究所;
【基金】：河北省卫生厅重大专项(20120025,20120242) “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题(2012ZX10003002-003)
【分类号】：R529.3

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