噬菌体Guo1复苏结核休眠菌的初步研究

发布时间：2018-05-11 01:13

本文选题：噬菌体Guo + 结核休眠菌　；参考：《南京医科大学学报(自然科学版)》2017年10期

【摘要】：目的:探索分枝杆菌噬菌体Guo1对结核休眠菌的复苏作用。方法:采用缺钾Sauton培养基培养对数生长期结核菌构建结核休眠菌模型;采用药敏检测、透射电子显微镜(电镜)观察和金胺O-尼罗红双荧光染色作为模型检测方法;采用菌落计数作为复苏的检测指标。结果:缺钾结核休眠菌模型构建成功。混合培养第1天时,空白对照组、上清组、TM4组、Guo1组菌落计数分别为(2.67±0.58)×10~2、(3.00±1.00)×10~2、(2.67±0.58)×10~2、(2.33±0.58)×10~2CFU/m L;培养第15天时,各组菌落计数分别为(7.33±1.53)×10~4、(6.67±2.08)×10~(10)、(3.00±1.00)×10~(10)、(3.33±1.53)×10~(10)CFU/m L。结论:分枝杆菌噬菌体Guo1能够复苏结核休眠菌。
[Abstract]:Objective: to explore the resuscitation effect of Mycobacterium bacteriophage Guo1 on Mycobacterium tuberculosis dormancy bacteria. Methods: the mycobacterium tuberculosis dormancy bacteria model was established by using potassium deficiency Sauton culture medium culture logarithmic growth phase tuberculous bacilli, drug sensitivity test, transmission electron microscope (TEM) observation and gold amine O- Nile red double fluorescence staining as the model detection method. Colony count was used as the index of resuscitation. Results: the model of dormancy bacteria was successfully constructed. On the first day of mixed culture, the colony counts of the control group and the TM4 group were 2.67 卤0.58) 脳 10 ~ (2 +) ~ (2) ~ (2) ~ (3) 卤1.00 卤1.00) 脳 10 ~ (2) ~ (2) ~ (2) C ~ (2) 卤0.58) 脳 10~2CFU/m ~ (2. 58) 脳 10~(10)CFU/m, respectively, and the colony counts of each group were 7.33 卤1.53 脳 10 ~ (4) + 6. 67 卤2.08) 脳 10 ~ (10) ~ (10) ~ (1. 00) 卤1.00) 脳 10 ~ (10) 10~(10)CFU/m ~ (3. 33 卤1. 53) 脳 10~(10)CFU/m 路L respectively on the 15th day after the culture, the colony counts of each group were 7. 33 卤1. 53) 脳 10 ~ (4), 6.67 卤2. 08) 脳 10 ~ 0. 00 卤1. 00) 脳 10 ~ (10) 10~(10)CFU/m. Conclusion: Mycobacterium bacteriophage Guo1 can resuscitate Mycobacterium tuberculosis dormancy bacteria.
【作者单位】：重庆医科大学附属第一医院呼吸与危重症医学科;
【基金】：国家自然科学基金面上项目(81570010) 国家临床重点专科专项(2012-649)
【分类号】：R52

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