贵阳市禽类相关外环境1013份标本中禽流感病毒检测结果分析

发布时间：2018-05-12 14:55

本文选题：禽流感病毒 + 外环境　；参考：《现代预防医学》2016年18期

【摘要】：目的了解贵阳市禽类相关外环境H5、H7、H9亚型禽流感病毒的污染情况,为贵阳市人禽流感的防控提供依据。方法 2015年10月-2016年3月,每月定期采集贵阳市禽类相关外环境中不同类型的标本共1 013份,采用Real-time PCR方法对环境标本进行Flu A、H5、H7、H9亚型禽流感病毒核酸检测,检测结果采用SPSS19.0软件进行分析。结果贵阳市禽类相关外环境Flu A阳性率为9.58%,H5、H9、H5+H9亚型禽流感阳性率分别为1.97%、4.94%、2.67%,未检测出H7亚型;不同监测点外环境Flu A阳性率差异有统计学意义(χ~2=66.741、P0.05),活禽交易市场、数字化蛋禽交易中心Flu A阳性率分别为8.99%、27.13%,禽类规模养殖场和禽类散养户集中地区未检出Flu A;不同类型标本Flu A阳性率差异无统计学意义(χ~2=5.245,P0.05),Flu A在案板表面擦拭物、粪便、笼具表面擦拭物、洗禽污水、禽类饮水及其他标本中的阳性率分别为7.78%、8.82%、9.77%、7.64%、14.77%、14.29%;非中心城区活禽交易市场Flu A阳性率高于中心城区(χ~2=50.534,P0.05)。结论贵阳市禽类相关外环境存在H5、H9亚型禽流感病毒污染,存在人感染高致病性禽流感的风险。
[Abstract]:Objective to investigate the contamination of avian influenza virus H5, H7, H9, and provide evidence for the prevention and control of human avian influenza in Guiyang. Methods from October 2015 to March 2016, a total of 1,013 samples of different types of avian influenza virus were collected regularly from Guiyang City from October 2015 to March 2016. Real-time PCR method was used to detect the nucleic acid of Flu Agna H5, H7H9 subtype avian influenza virus (Avian Influenza virus). The results were analyzed by SPSS19.0 software. Results the positive rate of Flu A in poultry related external environment in Guiyang was 9.58% and the positive rate of H5 H9 H5 H9 subtype avian influenza was 1.97% and 4.94%, respectively, and no H7 subtype was detected, and there were significant differences in Flu A positive rate between different monitoring points (蠂 26.741 P0.05, live poultry trade market). The positive rates of Flu A in digital egg poultry trading center were 8.99% and 27.13%, respectively. No Flu A positive rate was detected in poultry farms and poultry bulk farms, and there was no significant difference in Flu A positive rates among different types of specimens (蠂 2 + 25.245 P0.05FU A on the surface of the table, feces, etc. The positive rates of surface swabs, washing poultry sewage, drinking water and other specimens were 7.78 and 8.829.777.64 respectively. The positive rate of Flu A in the live poultry trading market was higher than that in the central city (蠂 ~ 2 ~ (2) + 50.534) (P _ (0.05). Conclusion the H5 / H9 subtype avian influenza virus is contaminated in the poultry environment of Guiyang city, and there is a risk of human infection with highly pathogenic avian influenza.
【作者单位】：贵州医科大学公共卫生学院;贵阳市疾病预防控制中心;
【分类号】：R511.7

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