河南省维生素D受体基因rs2222570和rs731236位点多态性与结核病的易感性

发布时间：2018-05-26 14:26

  本文选题：结核病 + 维生素D受体基因　； 参考：《郑州大学学报(医学版)》2017年05期

【摘要】：目的:探讨河南省人群维生素D受体(VDR)基因多态性和结核病易感性之间关系。方法:采用病例对照研究方法,病例组纳入260个结核病患者,并以年龄和性别相匹配纳入260名健康人作为对照组。采用PCR-SSCP法对VDR基因位点多态性进行分析。应用SPSS 19.0对数据进行统计分析,SHEsis在线分析2个位点单倍体型和结核病发病的关联,MDR软件3.0.2分析基因和环境交互作用对结核病发病的影响。结果:VDR基因rs2222570位点多态性与结核病发病风险相关,野生型C/C降低了结核病发病50%的风险(校正OR=0.501,95%CI:0.343~0.709),并且该位点等位基因C在对照组中高于病例组(校正OR=0.632,95%CI:0.493~0.868);而VDR基因的rs731236位点突变基因型C/C增加结核病发病风险(校正OR=2.001,95%CI:1.838~2.191)。VDR基因单倍体型CT、TT和TC与结核病发病相关[CT:OR(95%CI)=0.673(0.521~0.847),P0.001;TT:OR(95%CI)=1.332(1.043~1.711),P=0.032;TC:OR(95%CI)=6.561(2.366~18.143),P0.001]。MDR结果表明VDR基因rs2222570和rs731236位点多态性、吸烟、结核病患者密切接触史4个因素交互作用增加了结核病发病风险[OR(95%CI)=7.619(2.201~26.354),P0.001]。结论:VDR基因多态性与结核病发病风险相关,且VDR基因rs2222570和rs731236位点多态性、吸烟、结核病患者密切接触史存在交互作用。
[Abstract]:Objective: to investigate the relationship between vitamin D receptor (VDR) gene polymorphism and susceptibility to tuberculosis in Henan Province. Methods: a case-control study was conducted in 260 patients with tuberculosis and 260 healthy controls matched by age and sex. The polymorphism of VDR locus was analyzed by PCR-SSCP method. The data were statistically analyzed by SPSS 19.0. The correlation between haploid type of two loci and the incidence of tuberculosis was analyzed online by using SPSS 19.0. The effect of interaction between gene and environment on the incidence of tuberculosis was analyzed by using the software 3.0.2. Results the rs2222570 polymorphism of the VDR gene was associated with the risk of tuberculosis. Wild type C / C reduced the risk of TB incidence by 50% (adjusted ORO 0.501 / 95 CI: 0.343 / 0.709), and the allele C of this locus was higher in the control group than in the case group (adjusted ORO 0.63295 CI: 0.493C 0.868), while the rs731236 locus mutation genotype C / C of the VDR gene increased the risk of TB. The haploid type CTTT and TC of the VDR gene were associated with the incidence of tuberculosis. The interaction of four factors, smoking and close contact history of tuberculosis patients, increased the risk of tuberculosis. ConclusionVDR gene polymorphism is associated with the risk of tuberculosis, and the polymorphism of rs2222570 and rs731236 loci of VDR gene, smoking and close contact history of TB patients have interaction.
【作者单位】： 河南省疾病预防控制中心;
【基金】：“十二·五”科技重大专项课题2014ZX10003002 河南省卫生计生委普通科技攻关项目201702277
【分类号】：R52
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本文编号：1937564