不同来源金黄色葡萄球菌溶血素基因分布研究

发布时间：2018-05-30 00:32

本文选题：金黄色葡萄球菌 + 溶血素基因　；参考：《中国人兽共患病学报》2015年09期

【摘要】：目的了解金黄色葡萄球菌溶血素的携带情况,为金黄色葡萄球菌的防治及溯源提供依据。方法采用PCR扩增分别检测食品、临床患者和游泳池水等不同样本中hla、hlb、hlg和hld四种溶血素基因的分布情况,电泳检测扩增产物。结果317株金黄色葡萄球菌中,hla、hlb、hlg、hld四种溶血素基因的检出情况分别为85.5%、76.3%、89.6%、89.0%;在2008-2012年之间,四种溶血素基因的检出率无统计学差异。317株金黄色葡萄球菌中共有11种溶血素基因分布模式,检出率最高的模式为hlb/hlg,占71.9%,其次为hla/hld(70.3%),最少的是hla/hlb/hlg/hld(36.3%),不含溶血素基因的占2.2%。多数模式在3种来源菌株中的分布模式均有所不同。2008-2012年,每年同时检出hla、hlb、hlg、hld的菌株阳性率分别为45.7%、29.4%、32.5%、33.3%和32.1%。结论不同来源金黄色葡萄球菌中,溶血素基因携带及同时含有两种或者以上的情况普遍存在。
[Abstract]:Objective to investigate the carrying status of haemolysin in Staphylococcus aureus and to provide evidence for prevention and treatment of Staphylococcus aureus. Methods the distribution of hlahlbhlg and hld in different samples of food, clinical patients and swimming pool water were detected by PCR amplification, and the amplified products were detected by electrophoresis. Results in 317 strains of Staphylococcus aureus, the detection rate of four hemolysin genes was 85.55.576. 3 and 89.6and 89.0 respectively, and there was no statistical difference in the detection rate of four hemolysin genes between 2008 and 2012. There were 11 hemolysin gene distribution patterns in 317 strains of Staphylococcus aureus. The highest detection rate was in hlb / hlg, accounting for 71.9%, followed by hla / hldc70.3%, the lowest was hla / hlb / hlgr / hldrhldtir 36.3%, and those without hemolysin gene accounted for 2.2%. The distribution patterns of most of the patterns were different from 2008 to 2012. The positive rates of hla-hlbhlghld detected at the same time were 45.7% and 32.533% and 32.1g%, respectively. Conclusion in Staphylococcus aureus from different sources, hemolysin gene carries and contains two or more kinds of hemolysin gene.
【作者单位】：安徽马鞍山市疾病预防控制中心;河南省疾病预防控制中心;中国疾病预防控制中心传染病预防控制所;
【分类号】：R515

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