IL-10启动子区SNPs与青海地区藏族人群乙型肝炎病毒感染的相关研究

发布时间：2018-06-12 21:31

本文选题：青海地区 + 藏族　；参考：《青海大学》2013年硕士论文

【摘要】：目的：探讨青海地区藏族人群白介素-10（IL-10）基因多态性与乙型肝炎病毒(HBV)易感的相关性。本文主要研究IL-10-592、-1082位点基因单核苷酸多态性(SNP)与乙型病毒性肝炎易感性的关系。方法：采集青海地区藏族人群血样，根据乙肝五项及血清标记物检测结果抽取HBV感染患者血样250例作为病例组，抽取健康者外周血134例作为对照组。通过离心机离心获得单个核细胞及血清，由单个核细胞中提取基因组DNA，选取细胞因子IL-10-592、-1082位点进行多态性分析。最终通过统计学分析该基因在感染者与健康者之间有无显著差异。结果：1.在病例组中IL-10-592C/C、C/A、A/A位点各基因型频率分别为24.0%、52.0%、24.0%，与对照组IL-10-592C/C、C/A、A/A位点各基因型频率分别为23.9%、46.33%、29.9%比较，无显著差异，不具有统计学意义。在logistic多元回归分析中发现，与IL-10-592A/A基因型比较，携带IL-10-592C/A、C/C基因型的群体发生乙型病毒性肝炎的危险性分别为OR=1.398（95%CI=0.847-2.308），p=0.198，OR=1.250（95%CI=0.695-2.247），p=0.551，均无统计学意义。2.在病例组中IL-10-1082G/G、G/A、A/A位点各基因型频率分别为1.2%、23.2%、75.6%，与对照组IL-10-1082G/G、G/A、A/A位点各基因型频率分别为4.5%、19.4%、76.1%比较，无显著差异，不具备统计学意义。在logistic多元回归分析中发现，与IL-10-1082A/A比较，携带IL-10-1082G/A、G/G基因型的群体发生乙型病毒性肝炎的危险性分别为OR=1.204（95%CI=0.715-2.028），p=0.516，OR=1.270（95%CI=0.760-2.102），p=0.075，，均无统计学意义。结论：1.IL-10-592各基因型频率无显著差异。携带IL-10-592C/A、C/C基因型的群体与青海地区藏族人群乙型病毒性肝炎易感不相关。2.IL-10-1082各基因型频率无显著差异。携带IL-10-1082G/A、G/G基因型的群体与青海地区藏族人群乙型病毒性肝炎易感不相关。
[Abstract]:Objective: To investigate the correlation between the polymorphism of interleukin -10 (IL-10) gene and the susceptibility to hepatitis B virus (HBV) in the Tibetan population in Qinghai. This paper mainly studies the relationship between the single nucleotide polymorphisms (SNP) of the IL-10-592 and -1082 loci and the susceptibility of hepatitis B virus.
Methods: collecting blood samples from Tibetan population in Qinghai area, 250 cases of HBV infected patients were selected according to the five items of hepatitis B and the detection results of serum markers. 134 cases of peripheral blood from healthy people were selected as the control group. The single nucleus cells and serum were obtained by centrifuge centrifuge. The genomic DNA was extracted from single nuclear cells, and the cytokine was selected. The polymorphisms were analyzed by IL-10-592 and -1082 loci.
Results: 1. the genotype frequencies of IL-10-592C/C, C/A and A/A loci in the case group were 24%, 52%, 24% respectively. The genotype frequencies of IL-10-592C/C, C/A and A/A in the control group were 23.9%, 46.33%, 29.9%, respectively, and no significant difference was found. In logistic multivariate regression analysis, it was found to be compared with the IL-10-592A/A genotype, The risk of viral hepatitis B with IL-10-592C/A and C/C genotypes was OR=1.398 (95%CI=0.847-2.308), p=0.198, OR=1.250 (95%CI=0.695-2.247), p=0.551, and.2. was not statistically significant in the case group, IL-10-1082G/G, G/A, and the frequencies of the genotypes were 1.2%, 23.2%, and 75.6% respectively. The genotype frequencies of the A/A loci were 4.5%, 19.4%, and 76.1%, respectively, and no significant difference was found. In logistic multivariate regression analysis, the risk of viral hepatitis B with IL-10-1082G/A and G/G genotype was OR=1.204 (95%CI=0.715-2.028), p=0.516, OR=1.270 (95), respectively. %CI=0.760-2.102), p=0.075, no statistical significance.
Conclusion: there is no significant difference in the genotype frequencies of 1.IL-10-592. There is no significant difference in the genotype frequencies of IL-10-592C/A, C/C genotype and the susceptibility to viral hepatitis B in Qinghai Tibetan population. The population with IL-10-1082G/A, G/G genotype and the susceptibility to hepatitis B in the Tibetan people of Qinghai Haiti region are susceptible to viral hepatitis It's not related.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62

【参考文献】