侵袭性白假丝酵母菌感染小鼠模型中HMGB1的表达及意义

发布时间：2018-06-13 03:14

本文选题：白假丝酵母菌 + 感染　；参考：《免疫学杂志》2015年10期

【摘要】：目的研究高迁移率族蛋白B1(high mobility group box-1 protein,HMGB1)在侵袭性白假丝酵母菌感染小鼠模型中的表达及意义。方法 Balb/c小鼠腹腔注射环磷酰胺100 mg/(kg·d),连续3 d,检测小鼠血液中白细胞数量的变化。然后,通过尾静脉注射不同浓度的白假丝酵母菌悬液,选取合适的感染浓度并鉴定该浓度造模成功。将20只免疫抑制小鼠尾静脉注射白假丝酵母菌悬液,分别于感染后1、3、5和7 d处死小鼠,检测小鼠肝、肾功、C-反应蛋白等指标来明确小鼠病情严重程度,同时用Western blot检测小鼠肝、肺、肾组织中HMGB1的表达水平。结果本实验选取的侵袭性白假丝酵母菌感染的浓度为2×105/ml,组织培养和组织病理学方法均确定该浓度造模成功。生化结果显示,实验小鼠肝、肾功明显受损(总蛋白、球蛋白、谷草转氨酶、尿素、肌酐明显升高,胆碱酯酶明显降低)及C-反应蛋白明显升高。Western blot结果显示,实验组小鼠肝、肺、肾组织HMGB1的表达量均增加。肝组织HMGB1的表达量在第1天开始增加,第5天最明显(P0.01);肺和肾组织第5天开始明显增加(P0.01)。结论 HMGB1很可能在侵袭性白假丝酵母菌感染中起着重要作用。
[Abstract]:Objective to study the expression and significance of high mobility group protein B1 high mobility group box-1 protein HMGB1 in invasive Candida albicans infected mice. Methods Balb / c mice were intraperitoneally injected with cyclophosphamide 100 mg/(kg DX for 3 days. Then, different concentrations of Candida albicans suspension were injected into the tail vein, the appropriate concentration of infection was selected and the model was established successfully. Twenty immunosuppressive mice were injected with the suspension of Candida albicans via tail vein. The mice were killed on the 5th and 7th day after infection. The liver and kidney function C-reactive protein were detected to determine the severity of the disease in the mice, and the liver of the mice was detected by Western blot. Expression of HMGB1 in lung and kidney. Results the concentration of invasive Candida albicans infection was 2 脳 10 5 / ml, which was confirmed by tissue culture and histopathology. The results of biochemistry showed that the liver and kidney function of the experimental mice were obviously damaged (total protein, globulin, glutamic oxaloacetic transaminase, urea, creatinine, cholinesterase were obviously increased, and the C-reactive protein was obviously increased. Western blot showed that the liver of the experimental group was obviously increased. The expression of HMGB1 in lung and kidney was increased. The expression of HMGB1 in liver tissue began to increase on day 1, and the expression of HMGB1 in liver tissue increased significantly on day 5, and the expression of HMGB1 protein in lung and kidney tissues increased significantly on day 5. Conclusion HMGB1 may play an important role in invasive Candida albicans infection.
【作者单位】：重庆医科大学附属第二医院病毒性肝炎研究所;重庆医科大学附属第二医院临床检验中心;重庆医科大学附属第二医院肝胆外科;
【基金】：国家自然科学基金(81171543)
【分类号】：R519

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