无偿献血者HCV核心区氨基酸置换情况分析

发布时间：2018-06-19 00:46

本文选题：HCV基因型 + HCV核心区　；参考：《中国输血杂志》2017年09期

【摘要】：目的研究感染HCV的无症状献血者基因型和1b基因型的核心区氨基酸置换情况。方法用巢式PCR方法扩增91个HCV RNA阳性献血者标本,根据直接测序得到的序列用Mega6.0软件进行序列比对和进化树构建,分析基因型和氨基酸置换情况。结果在78个测序结果清晰的样品中,66个为基因型1b(84.6%),其他基因型为1a(1,1.3%)、2a(7,8.9%)、3b(2,2.6%)和6a(2,2.6%)。62个1b样品的核心区蛋白(aa68-120)发生17处氨基酸置换。第70位R70Q、R70P和R70H各1例;第91位L91M置换非常普遍,为87.9%。结论本研究中献血者感染的HCV基因型主要为1型,尤其是1b为优势基因型。首次发现中国献血者感染的HCV核心区蛋白的氨基酸置换具有独特性。
[Abstract]:Objective to study the amino acid exchange in core region of asymptomatic blood donors infected with HCV and genotype 1b. Methods 91 HCV RNA positive blood donors were amplified by nested PCR. The sequence alignment and phylogenetic tree construction were carried out by Mega6.0 software according to the sequence obtained by direct sequencing. The genotype and amino acid replacement were analyzed. Results among the 78 samples with clear sequencing results, 66 were genotypes 1b, 84.6, and the others were 1a, 1. 3, 1. 3, 78.9, 78.9) and 6a, 22. 6 and 62 1b, respectively. 17 amino acid substitutions occurred in the core region protein aa68-120 of 62 1b samples. There were 1 case of R70QN R70P and 1 case of R70H at the 70th position, and 87.9% of L91M replacement at the 91st position. Conclusion the HCV genotype of blood donors in this study was mainly genotype 1, especially 1b. It is the first time that the amino acid replacement of HCV core protein infected by Chinese blood donors is unique.
【作者单位】：江苏省血液中心;
【基金】：江苏省“六大人才高峰”项目(2014-WSN-002) 江苏省第五期“333高层次人才培养工程”资助项目(LGY2016045)
【分类号】：R512.63

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