梅毒患者外周血调节性B细胞亚群在治疗前后的变化研究

发布时间：2018-06-26 02:09

本文选题：梅毒患者 + CD+CDhiCDhiB细胞　；参考：《中国艾滋病性病》2017年10期

【摘要】：目的探讨未经治疗的一期梅毒、二期梅毒和隐性梅毒患者外周血调节性B细胞亚群(CD19+CD24hi CD38hi B细胞、CD19+CD24hi CD27+B细胞)的百分比,及其在治疗后的变化情况。方法根据《梅毒诊疗指南(2014)》标准,2016年9月至2017年3月,在北京大学地坛医院教学医院皮肤性病科门诊的43例梅毒患者及8例健康对照。在治疗前及苄星青霉素治疗后2个月进行随访,对每次随访患者利用流式细胞技术检测外周血CD19+CD24hi CD38hi B细胞、CD19+CD24hi CD27+B细胞的百分比。结果 43例梅毒患者中,一期梅毒13例,二期梅毒16例,隐性梅毒14例。与健康对照组相比,未经治疗的一期、二期梅毒患者外周血CD19+CD24hi CD38hi B细胞百分比显著升高,CD19+CD24hi CD27+B细胞百分比显著降低,差异均有统计学意义(P0.05);隐性梅毒患者与健康对照之间均无明显差异(P0.05)。治疗后,一期梅毒、二期梅毒及隐性梅毒患者外周血CD19+CD24hi CD38hi B细胞百分比均显著降低,差异均有统计学意义(P0.05);而CD19+CD24hi CD27+B细胞百分比均无明显改变,差异均无统计学意义(P0.05)。相关性分析结果:梅毒患者外周血CD19+CD24hi CD38hi B细胞百分比与TRUST滴度呈正相关(P0.01);CD19+CD24hi CD27+B细胞百分比与TRUST滴度呈负相关(P0.01)。结论CD19+CD24hi CD38hi B细胞有可能是一期、二期梅毒患者的重要免疫学变化指标之一。苄星青霉素治疗能快速有效降低一期、二期梅毒患者外周血CD19+CD24hi CD38hi B细胞百分比。
[Abstract]:Objective to investigate the percentage of peripheral blood regulatory B cell subsets (CD19 CD24hi CD38hi B cell) in untreated patients with primary syphilis, secondary syphilis and recessive syphilis, and their changes after treatment. Methods according to the guidelines for syphilis diagnosis and treatment (2014), from September 2016 to March 2017, 43 syphilis patients and 8 healthy controls were enrolled in the Department of Dermatology and venereal Diseases Department, The Temple of Earth Hospital of Peking University. The percentage of CD19 CD24hi CD38hi B cells and CD19 CD24hi CD27 B cells in peripheral blood were detected by flow cytometry before and 2 months after benzylpenicillin treatment. Results among 43 cases of syphilis, 13 cases were primary syphilis, 16 cases were secondary syphilis and 14 cases were recessive syphilis. Compared with the control group, the percentage of CD19 CD24hi CD38hi B cells in peripheral blood of patients with secondary syphilis was significantly increased and the percentage of CD19 CD24hi CD27 B cells significantly decreased in patients with secondary syphilis. The differences were statistically significant (P0.05); there was no significant difference between recessive syphilis patients and healthy controls (P0.05). After treatment, the percentage of CD19 CD24hi CD38hi B cells in peripheral blood of patients with primary syphilis, secondary syphilis and recessive syphilis decreased significantly (P0.05), while the percentage of CD19 CD24hi CD27 B cells did not change significantly (P0.05). The results of correlation analysis showed that the percentage of CD19 CD24hi CD38hi B cells in peripheral blood of syphilis patients was positively correlated with trust titer (P0.01) and the percentage of CD19 CD24hi CD27B cells was negatively correlated with trust titer (P0.01). Conclusion CD19 CD24hi CD38hi B cells may be one of the important immunological markers in patients with primary and secondary syphilis. Benzylpenicillin treatment could reduce the percentage of CD19 CD24hi CD38hi B cells in peripheral blood of patients with secondary syphilis.
【作者单位】：北京大学地坛医院教学医院;首都医科大学附属北京地坛医院感染病科国家临床重点专科北京市中医管理局十二五重点建设专科;
【基金】：北京市卫生系统高层次卫生技术人才培养计划(2011-3-080)~~
【分类号】：R759.1

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