两种核酸检测法在副溶血性弧菌耐热直接溶血素和不耐热溶血毒素中的应用评估

发布时间：2018-07-03 03:24

本文选题：副溶血性弧菌 + 核酸检测法　；参考：《疾病监测》2016年09期

【摘要】：目的比较CPA-核酸试纸条法与荧光定量PCR法检测副溶血性弧菌(VP)的tlh种特异性基因和tdh毒力基因的灵敏度与特异性。方法采用CPA-核酸试纸条法和荧光定量PCR法同时对655株不同来源的VP菌株进行tlh、tdh基因检测。结果 CPA-核酸试纸条法与荧光定量PCR法检测结果相同,VP菌株的tlh基因检测阳性率均为100%(655/655)。两种方法对tdh基因的检测结果也完全一致,tdh基因临床株与海产品株阳性检测率均分别为95.8%(207/216)与2.27%(10/439)。CPA-核酸试纸条法对tlh、tdh基因检测具有高度特异性,灵敏度可达6.2×103cfu/ml菌液,从核酸制备至完成检测最快仅需1 h。结论 VP临床株与涉水产品分离株普遍携带tlh种特异性基因,而tdh毒力基因主要存在于临床标本而在涉水产品株极少存在,可采用敏感、特异的CPA-核酸试纸条法,做VP腹泻患者的床边诊断以及食物中毒事故现场的快速检测。
[Abstract]:Objective to compare the sensitivity and specificity of tlh specific gene and tdh virulence gene of Vibrio parahaemolyticus (VP) by CPA- nucleic acid test strip method and fluorescence quantitative PCR method. Methods using CPA- nucleic acid test strip method and fluorescence quantitative PCR method, 655 VP strains from different sources were detected for tlhmtdh gene at the same time. Results the positive rate of tlh gene was 100% (655 / 655). The positive detection rates of tdh gene in clinical strains and seafood strains were 95.8% (20.7 / 216) and 2.27% (10 / 439), respectively. CPA-DNA strip assay was highly specific for the detection of tdh gene, and the sensitivity was 6.2 脳 103cfu/ml. From nucleic acid preparation to complete detection, the fastest time is 1 hour. Conclusion VP clinical strains and water-wading product isolates generally carry tlh species-specific genes, while tdh virulence genes are mainly present in clinical specimens and rarely exist in wading product strains, so the sensitive and specific CPA-nucleic acid test strip method can be used. To do the bedside diagnosis of VP diarrhea patients and the rapid detection of food poisoning accident site.
【作者单位】：杭州市江干区疾病预防控制中心;浙江省疾病预防控制中心;杭州市下城区疾病预防控制中心;杭州市上城区疾病预防控制中心;
【基金】：杭州市社会发展科研攻关项目(No.20130533B46) 浙江省医药卫生科技计划(No.2014ZHA003)~~
【分类号】：R516.3;R440

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