雷丸及吡喹酮对猬迭宫绦虫裂头蚴感染性及超微结构的影响

发布时间：2018-07-03 06:30

本文选题：雷丸 + 吡喹酮　；参考：《中国寄生虫学与寄生虫病杂志》2015年01期

【摘要】：目的了解雷丸及吡喹酮对猬迭宫绦虫裂头蚴感染性及超微结构的影响。方法从黑斑蛙体内检获裂头蚴,用20、40和80 mg/ml雷丸粉末混悬培养液以及20、80和320μg/ml吡喹酮培养液分别体外培养裂头蚴4、12和24 h,单纯培养液培养4、12和24 h为感染对照组。168只昆明小鼠均分为21组(每组8只),每组小鼠经口感染相应条件培养后的裂头蚴各5条/鼠。感染后1周剖杀各组小鼠,计数每鼠裂头蚴检出数,并计算每组小鼠的减虫率。扫描电镜和透射电镜分别观察不同培养处理后的裂头蚴超微结构变化。结果小鼠感染经40和80 mg/ml雷丸粉末混悬液分别培养4、12和24 h的裂头蚴1周后,每组小鼠裂头蚴平均检出数分别为1.6、1.0和0.3条,0.3、0和0条,均显著低于对照组(4.1、3.5和3.3条)(P0.05),且两组间差异有统计学意义(P0.05);减虫率分别为60.0%、71.4%和90.1%,92.7%、100%和100%,且两组间差异有统计学意义(P0.05)。小鼠感染经320μg/ml吡喹酮溶液分别培养4、12和24 h的裂头蚴1周后,每组小鼠裂头蚴平均检出数分别为1.9、1.3和0.4条,与对照组间差异均有统计学意义(P0.05);减虫率分别为53.7%、62.9%和87.9%,显著高于20μg/ml吡喹酮处理组的14.6%、2.9%和6.1%以及80μg/ml吡喹酮处理组的24.4%、17.1%和24.2%(P0.05)。扫描电镜和透射电镜观察可见,经40 mg/ml雷丸粉末混悬液培养4 h以及320μg/ml吡喹酮溶液培养4和12 h后,裂头蚴虫体出现轻度挛缩;经40 mg/ml雷丸粉末混悬液培养12和24 h,虫体微毛凝集、融合或脱落,质膜破裂,石灰小体释出,皮质组织受损;经80 mg/ml雷丸粉末混悬液培养4~12 h,虫体损害逐渐加重,至24 h,裂头蚴组织结构严重受损,无法辨清;经320μg/ml吡喹酮溶液培养24 h,虫体前端挛缩明显,质膜水肿、泡状隆起,石灰小体形态改变,糖原颗粒耗损,分泌颗粒增加及焰细胞核质浓缩。经20 mg/ml雷丸粉末混悬液、20和80μg/ml吡喹酮溶液培养4、12和24 h的裂头蚴虫体与对照组相比,超微结构无明显改变。结论随着吡喹酮和雷丸体外培养浓度的增加以及时间的延长,裂头蚴对小鼠的感染性逐渐降低,并引起虫体广泛的组织结构损伤;雷丸的作用较吡喹酮明显。
[Abstract]:Objective to investigate the effects of Lei Pill and praziquantel on the infection and ultrastructure of tapeworm. Methods the caterpillars were seized from the body of the black spot frog. The mice were cultured in 20, 40 and 80 mg/ml powder suspension medium and 20 80 and 320 渭 g/ml praziquantel solution for 12 and 24 hours, respectively. The mice were divided into 21 groups (8 in each group). Each group of mice was infected by oral infection with 5 caterpillars per mouse. One week after infection, the mice in each group were dissected, the number of mitomycaria per mouse was counted, and the rate of worm reduction in each group was calculated. The ultrastructural changes of mitomycaria were observed by SEM and TEM, respectively. Results after the mice were infected by 40 and 80 mg/ml powder suspension of Lei Pill for 1 week, the average detectable numbers of mitomycaria in each group were 1. 6% and 0. 3%, respectively, after cultured for 12 h and 24 h, respectively, in each group, the number of mitomycaria in each group was 0. 30% and 0. 3%, respectively. There was significant difference between the two groups (P0.05), the worm reduction rate was 60.071.4% and 90.1%, respectively, and there was significant difference between the two groups (P0.05). After the mice were incubated with 320 渭 g/ml praziquantel solution for 1 week for 12 and 24 hours respectively, the average detectable numbers of mitomycaria in each group were 1.9 and 0.3,0.4, respectively. There were significant differences between the control group and the control group (P0.05), the worm reduction rates were 53.7% and 87.9%, respectively, which were significantly higher than those in the 20 渭 g/ml praziquantel treatment group (14.6g% and 6.1%) and the 80 渭 g/ml praziquantel group (24.4% and 24.2%, P0.05). Scanning electron microscopy and transmission electron microscopy showed that after 40 mg/ml powder suspension culture for 4 h and 320 渭 g/ml praziquantel solution for 4 h and 12 h, slight contracture was observed. After 40 mg/ml Lei Pill powder suspension culture for 12 and 24 hours, the insect body was agglutinated, fused or shed, the plasma membrane was broken, the lime body was released and the cortical tissue was damaged, and after 80 mg/ml Lei Wan powder suspension culture for 412 h, the damage of the insect body gradually increased. At 24 h, the tissue structure of mitomycaria was seriously damaged and could not be identified, and after cultured in 320 渭 g/ml praziquantel solution for 24 h, the contracture of the front end of the worm was obvious, the membrane edema, the bulge, the morphology of lime bodies and the loss of glycogen granules were observed. The secretory granules were increased and the nuclear cytoplasm of the flame was concentrated. Compared with the control group, the ultrastructure of 20 mg/ml Lei Wan powder suspension and 80 渭 g/ml praziquantel solution cultured for 4 h and 24 h showed no obvious changes. Conclusion with the increase of culture concentration and time of praziquantel and Lei Pill in vitro, the infectivity of mitomycaria to mice gradually decreased and caused extensive tissue structure damage, and the effect of Lei Wan was more obvious than that of praziquantel.
【作者单位】：贵阳医学院人体寄生虫学教研室;
【基金】：贵州省科技厅基金项目[黔科合J字(2012)2042号]~~
【分类号】：R532.3

【参考文献】

相关期刊论文前10条

1 每乐;刘纯;赵兵;杨红超;蒋璐鹭;王卫卫;巩江;倪士峰;;雷丸药学研究概况[J];安徽农业科学;2013年10期

2 兰婧;陈艳;陶梓璐;裘学丽;徐婧;;实验小鼠体内曼氏裂头蚴移行途径及分布的研究[J];四川动物;2014年06期

3 刘自逵;刘国华;戴荣四;刘伟;李芬;胡涛;刘毅;;湖南省猬迭宫绦虫的线粒体cox1和nad1基因的序列测定及种系发育分析[J];畜牧兽医学报;2010年04期

4 李楠;蔺西萌;崔晶;王明明;井丰军;祁欣;王莉;王中全;;不同剂量吡喹酮治疗曼氏裂头蚴感染小鼠的疗效观察[J];中国血吸虫病防治杂志;2010年01期

5 胡珊梅;李玲玲;肖新月;陈洁;;雷丸药材定性和定量分析方法研究[J];药物分析杂志;2010年09期

6 许明峰;沈莲清;王奎武;;雷丸化学成分的研究[J];中草药;2011年02期

7 郑灏;程显隆;肖新月;魏锋;付凌燕;林玉莲;;雷丸药材中多糖及总糖定量分析方法研究[J];中国药事;2011年09期

8 周立华;许勤勤;张一琼;周振兴;管文军;李永泉;;雷丸的中性金属蛋白酶的分离纯化、酶学性质及体外驱虫活性(英文)[J];Chinese Journal of Chemical Engineering;2010年01期

9 赵冠宏,许炽标,冯曼玲,尤金英,郭毛娣,李焕娄;雷丸蛋白酶体外抗猪囊尾蚴组织学变化的观察[J];中国寄生虫学与寄生虫病杂志;1998年02期

10 祁欣;姜鹏;崔晶;田翔宇;陈梦欢;范思洋;陈雨;张玺;王中全;;吡喹酮对曼氏裂头蚴感染小鼠治疗效果的进一步研究[J];中国病原生物学杂志;2013年08期

【共引文献】

相关期刊论文前10条

1 丁义玲;夏鹏;胡东海;皇甫政彤;王伟;黄隆福;陈瑞娟;;荆州市蔬菜携带土源性线虫情况的调查[J];安徽农业科学;2008年34期

2 丁义玲;夏鹏;张晓方;李侃;;荆州市农贸市场食源性寄生虫病原学调查[J];安徽农业科学;2010年24期

3 孙新;陈兴智;杨小迪;方强;胡守锋;夏惠;张美华;王雪梅;王媛媛;;我国首例人体司氏伯特绦虫病——节片形态及流行病学意义[J];蚌埠医学院学报;2008年06期

4 张潞渝;张静;叶彬;武卫华;;两种不同培养基体外培养阴道毛滴虫的增殖效果比较[J];重庆医学;2011年33期

5 贾志江;杨红梅;刘娣琴;;犬传绦虫蚴病流行病学分析及综合防治技术[J];当代畜牧;2010年04期

6 农子军;覃都娥;莫刚;蒋莉萍;;杀螨霜治疗蠕形螨病的实验和临床研究[J];当代医学;2009年28期

7 张迎春;漆一鸣;;苯醚威对印鼠客蚤的毒力试验[J];地方病通报;2008年02期

8 郑莉莉;崔昱;陈凤义;戴晓冬;秦元华;任一鑫;;大连市6例微小膜壳绦虫感染及其病例分析[J];大连医科大学学报;2006年04期

9 郑莉莉;张凤;秦元华;李永波;张斌;陈凤义;崔昱;;大连瓦房店市区肝吸虫病流行病学调查[J];大连医科大学学报;2010年02期

10 舒琴;张锡林;张莉林;荣贵贤;;人体感染眼结膜吸吮线虫病1例[J];第三军医大学学报;2008年16期

相关期刊论文前10条

1 王宏;程显好;刘强;樊飞跃;;雷丸研究进展[J];安徽农业科学;2008年35期

2 汤军,黄琦,徐志瑛,蔡宛如,钱华,宋康;麦冬多糖的提取分离与初步分析[J];浙江中医学院学报;1999年01期

3 蒋柏荣;陆红星;章国强;汪新标;;雷丸驱虫灵驱杀鸭胃肠道绦虫的临床效果试验[J];浙江畜牧兽医;2009年02期

4 刘经平,刘力;中药雷丸注射液抗小白鼠S_(180)后瘤块的组织学观察[J];赣南医学院学报;1988年01期

5 陈芳;周侣艳;;苯酚标准溶液的稳定性探讨[J];环境研究与监测;2004年03期

6 肖树华;抗血吸虫及抗绦虫新药吡喹酮的实验研究[J];国外医学参考资料(寄生虫病分册);1978年06期

7 李德兴;;苯并咪唑类抗蠕虫药物[J];国外医学(寄生虫病分册);1991年05期

8 刘进新;中药雷丸不同剂量驱杀鸡绦虫效果观察[J];贵州畜牧兽医;1996年02期

9 方根华;;雷丸不应煎服[J];安庆医学;2000年04期

10 梁菊芬;何婷;陈萍;;雷丸片质量标准研究[J];大众科技;2012年01期

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