福建省1例不明原因肺炎病例中甲型H1N1流感病毒的全基因组特征分析

发布时间：2018-07-25 16:02

【摘要】：目的了解1例不明原因肺炎病例中甲型H1N1流感病毒的生物学特性和变异情况,为临床治疗与疫情防控提供依据。方法利用MDCK细胞分离不明原因肺炎病例中的甲型H1N1流感病毒,分离的毒株经全基因组测序后分析其抗原性、致病性和耐药性等特征。结果从该病例的咽拭子标本中分离得到1株甲型H1N1流感病毒并命名为A/FujianGulou/SWL64/2016(H1N1),其8个节段的核苷酸和氨基酸序列与A/California/07/2009(H1N1)疫苗株的相似性分别为96.9%~98.9%和96.7%~99.5%。氨基酸序列分析显示HA蛋白共有18个位点发生突变,其中K163Q、S185T、S203T和D222N变异涉及到3个不同的抗原位点,提示病毒发生抗原漂移;与受体结合位点相关的D222N突变还提示病毒感染下呼吸道的能力增强。耐药性分析显示该病毒对金刚烷胺耐药,对达菲仍然敏感。结论本次研究的甲型H1N1流感病毒具有抗原漂移现象,且具备引发重症肺炎的分子特征,应进一步加强监测,为疫情防控奠定基础。
[Abstract]:Objective to investigate the biological characteristics and variation of influenza A (H1N1) virus in a case of unknown pneumonia and to provide evidence for clinical treatment and prevention and control. Methods MDCK cells were used to isolate influenza A (H1N1) virus from patients with unexplained pneumonia. The antigenicity, pathogenicity and drug resistance of the isolates were analyzed by genomic sequencing. Results A strain of A H1N1 influenza virus named A/FujianGulou/SWL64/2016 (H1N1) was isolated from the throat swabs of this case. The similarity of nucleotide and amino acid sequences between the 8 segments of the virus and A/California/07/2009 (H1N1) vaccine strain was 96.9% and 96.799.5%, respectively. Amino acid sequence analysis showed that there were 18 mutations in HA protein, among which K163QN S185TnS203T and D222N mutations were involved in three different antigenic sites, suggesting that the virus had antigen drift. The D 222N mutation associated with receptor binding sites also suggests an increased ability of the virus to infect lower respiratory tract. Drug resistance analysis showed that the virus was resistant to amantadine and remained sensitive to Tamiflu. Conclusion the influenza A H1N1 virus in this study has the phenomenon of antigen drift and the molecular characteristics of causing severe pneumonia. The surveillance should be further strengthened to lay the foundation for the prevention and control of the epidemic situation.
【作者单位】：福建省疾病预防控制中心福建省人兽共患病研究重点实验室;福建医科大学公共卫生学院;福建省国际旅行卫生保健中心;
【基金】：福建省自然科学基金(No.2015J01152) 福建省医学创新课题(No.2014-CX-9)资助福建省卫生系统中青年骨干人才培养项目(No.2015-ZQN-ZD-10)~~
【分类号】：R511.7

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