广东首例苏里南输入性寨卡病毒感染病例的实验室检测

发布时间：2018-07-27 18:28

【摘要】：目的对从广州白云国际机场口岸入境的一例自苏里南归国发热人员进行寨卡病毒实验室检测。方法采集发热人员的血液和唾液样本,同时使用两种寨卡病毒实时荧光RT-PCR试剂进行寨卡病毒核酸检测。RT-PCR扩增寨卡病毒部分基因片段,并进行序列测定和同源性比较。基于扩增片段构建系统进化树,分析输入性病例的来源。结果实时荧光RT-PCR结果显示,该病例血清样本寨卡病毒核酸弱阳性、唾液样本寨卡病毒核酸阳性。RT-PCR扩增出预期大小约1.5Kb片段,测序结果表明该片段与巴西寨卡病毒株(GenBank号KX197250)相应序列的同源性为99%。系统进化树显示该病毒属亚洲谱系。结论根据患者流行病学史、临床表现和病例标本核酸检测情况,确诊该病例为广东首例从苏里南地区输入性寨卡病例。
[Abstract]:Objective to detect Zika virus in a case of returning fever from Suriname in Guangzhou Baiyun International Airport. Methods Blood and saliva samples of febrile persons were collected and sequenced and homology were compared by using two kinds of real time fluorescent RT-PCR reagent to detect Zika virus nucleic acid and amplify partial DNA fragments of Zika virus by reverse transcription-polymerase chain reaction (RT-PCR). Phylogenetic tree was constructed based on amplified fragments and the origin of imported cases was analyzed. Results the results of real-time fluorescence RT-PCR showed that Zika virus nucleic acid was weakly positive in serum and saliva samples were positive for Zika virus nucleic acid. RT-PCR amplified the expected size of Zika virus 1.5Kb fragment. The sequencing results showed that the sequence of the fragment was 99% homology with the corresponding sequence of Brazilian Zika virus strain (GenBank KX197250). The phylogenetic tree shows that the virus belongs to the Asian lineage. Conclusion according to the epidemiological history, clinical manifestation and nucleic acid detection, the case was confirmed as the first imported Zika case from Suriname in Guangdong province.
【作者单位】：广东出入境检验检疫局检验检疫技术中心卫生检疫实验室;广东出入境检验检疫局;
【基金】：广东省科技计划项目(No.2016A020247001) 广东检验检疫局科技计划项目(No.2016GDK58)联合资助~~
【分类号】：R440;R511

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