乙型肝炎病毒感染与ABCB4基因及胆囊胆固醇结石形成的关系

发布时间：2018-08-24 15:29

【摘要】：目的探讨乙型肝炎(乙肝)病毒感染对ABCB4基因的影响以及与胆囊胆固醇结石形成的相关性。方法选择行胆囊切除的胆囊结石患者48例,HBs Ag阳性28例为观察组,HBs Ag阴性20例为对照组。两组胆囊分别做HE染色及免疫组化S-P法检测乙肝病毒表面抗原和核心抗原,应用Real-time PCR技术检测两组的ABCB4基因表达水平。结果 HBs Ag在胆囊黏膜组织中的表达,观察组阳性率为57.1%,对照组全为阴性,两组比较差异有统计学意义(P0.05)。HBc Ag在胆囊黏膜中的表达,观察组阳性率为28.6%,对照组全为阴性,两组比较差异有统计学意义(P0.05)。观察组ABCB4基因mRNA表达(0.909±0.059)较低,对照组ABCB4基因mRNA表达(1.149±0.039)较高,两组比较差异有统计学意义(t=-11.062,P0.05)。结论乙肝病毒感染患者胆囊ABCB4基因的表达下调,可能导致胆囊胆固醇结石的形成。
[Abstract]:Objective to investigate the effect of hepatitis B virus infection on ABCB4 gene and its correlation with cholesterol gallstone formation. Methods A total of 48 patients with cholecystolithiasis underwent cholecystectomy were divided into two groups: control group (n = 20). Hepatitis B virus surface antigen and core antigen were detected by HE staining and S-P immunohistochemical method. ABCB4 gene expression was detected by Real-time PCR technique. Results the positive rate of HBs Ag in gallbladder mucosa was 57.1 in the observation group and negative in the control group. The difference between the two groups was statistically significant (P0.05). The positive rate of HBc Ag in the observation group was 28.6g and that in the control group was negative. The difference between the two groups was statistically significant (P0.05). The mRNA expression of ABCB4 gene in the observation group (0.909 卤0.059) was lower than that in the control group (1.149 卤0.039). The difference between the two groups was statistically significant (P0.05). Conclusion the down-regulated expression of ABCB4 gene may lead to the formation of cholecystolithiasis in patients with hepatitis B virus infection.
【作者单位】：广州医科大学附属第一医院海印院区腹部外科;
【基金】：2011年广东省第一批科学事业费计划项目(编号:2011B031800131)
【分类号】：R512.62;R575.6

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