HBV感染对肝脏自然杀伤细胞和固有淋巴样细胞22的影响
发布时间：2018-08-25 14:24
【摘要】：目的探讨HBV感染对肝脏自然杀伤细胞(NK细胞)和固有淋巴样细胞(ILC)22的影响,为阐明HBV感染诱导机体固有免疫应答的机制提供理论和实验依据。方法选取6~8周龄雄性BALB/c小鼠10只,随机分为实验组和对照组,每组各5只。实验组小鼠经尾静脉高压注射含10μg HBV全基因组质粒的生理盐水(体积相当于9%小鼠体质量);对照组小鼠仅注射生理盐水。4 d后处死小鼠,分离肝脏内淋巴细胞,应用流式细胞术检测肝脏内淋巴细胞中NK细胞亚群和ILC22细胞亚群的比例。组间比较采用t检验。结果高压尾静脉注射HBV全基因组质粒可诱导小鼠产生高水平的HBs Ag和HBe Ag,伴有血清ALT水平升高。实验组小鼠肝脏内NK细胞的比例较对照组小鼠显著升高[(25.90±4.92)%vs(12.98±2.13)%,t=3.811,P=0.003],但CD127+NK细胞和CD127-NK细胞亚群在NK细胞中所占比例在2组之间差异均无统计学意义(P值均0.05)。实验组小鼠肝脏内NKp46+ILC22细胞亚群比例较对照组明显升高[(36.05±6.85)%vs(10.22±3.54)%,t=7.372,P0.001],但NKp46-ILC22细胞亚群的比例在2组之间差异无统计学意义(P0.05)。结论 HBV感染可诱导肝脏内NK细胞和NKp46+ILC22水平升高,从而促进肝脏固有免疫应答。
[Abstract]:Objective to investigate the effects of HBV infection on natural killer cells (NK cells) and lymphoid like cells (ILC) 22) of the liver, and to provide theoretical and experimental evidence for elucidating the mechanism of the innate immune response induced by HBV infection. Methods Ten male BALB/c mice aged 6 and 8 weeks were randomly divided into experimental group and control group with 5 mice in each group. The mice in the experimental group were injected with saline containing 10 渭 g HBV genome plasmid through caudal vein hyperbaric injection (volume equivalent to 9% of the body mass of mice), and the control group mice were killed after injection of saline only for 4 days, and the lymphocytes in the liver were isolated. The proportion of NK cell subsets and ILC22 cell subsets in liver lymphocytes was detected by flow cytometry. T test was used for comparison between groups. Results High pressure tail vein injection of HBV genome plasmid could induce high levels of HBs Ag and HBe Ag, with elevated serum ALT level in mice. The percentage of NK cells in the liver of the experimental group was significantly higher than that of the control group [(25.90 卤4.92) vs (12.98 卤2.13)], but the proportion of CD127 NK cells and CD127-NK subsets in the NK cells was not significantly different between the two groups (P < 0. 05). The percentage of NKp46 ILC22 cell subsets in the liver of the experimental group was significantly higher than that of the control group [(36.05 卤6.85) vs (10.22 卤3.54)], but there was no significant difference in the proportion of NKp46-ILC22 cell subsets between the two groups (P0.05). Conclusion HBV infection can induce the increase of NK cells and NKp46 ILC22 levels in the liver and promote the inherent immune response of the liver.
【作者单位】： 第四军医大学唐都医院全军感染病诊疗中心;
【基金】：国家自然科学基金资助项目(31200650;81671555) 唐都医院后备人才课题
【分类号】：R512.62
本文编号：2203145
[Abstract]:Objective to investigate the effects of HBV infection on natural killer cells (NK cells) and lymphoid like cells (ILC) 22) of the liver, and to provide theoretical and experimental evidence for elucidating the mechanism of the innate immune response induced by HBV infection. Methods Ten male BALB/c mice aged 6 and 8 weeks were randomly divided into experimental group and control group with 5 mice in each group. The mice in the experimental group were injected with saline containing 10 渭 g HBV genome plasmid through caudal vein hyperbaric injection (volume equivalent to 9% of the body mass of mice), and the control group mice were killed after injection of saline only for 4 days, and the lymphocytes in the liver were isolated. The proportion of NK cell subsets and ILC22 cell subsets in liver lymphocytes was detected by flow cytometry. T test was used for comparison between groups. Results High pressure tail vein injection of HBV genome plasmid could induce high levels of HBs Ag and HBe Ag, with elevated serum ALT level in mice. The percentage of NK cells in the liver of the experimental group was significantly higher than that of the control group [(25.90 卤4.92) vs (12.98 卤2.13)], but the proportion of CD127 NK cells and CD127-NK subsets in the NK cells was not significantly different between the two groups (P < 0. 05). The percentage of NKp46 ILC22 cell subsets in the liver of the experimental group was significantly higher than that of the control group [(36.05 卤6.85) vs (10.22 卤3.54)], but there was no significant difference in the proportion of NKp46-ILC22 cell subsets between the two groups (P0.05). Conclusion HBV infection can induce the increase of NK cells and NKp46 ILC22 levels in the liver and promote the inherent immune response of the liver.
【作者单位】： 第四军医大学唐都医院全军感染病诊疗中心;
【基金】：国家自然科学基金资助项目(31200650;81671555) 唐都医院后备人才课题
【分类号】：R512.62
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