地塞米松对细菌性脑膜炎大鼠脑组织咬合蛋白表达的影响

发布时间：2018-10-10 12:39

【摘要】：目的观察地塞米松对细菌性脑膜炎大鼠脑组织咬合蛋白(occludin)表达的干预作用。方法 3周龄SD大鼠40只随机分为对照组、模型组、CTRX组和CTRX+DEXA组,除对照组外,其他各组均制作细菌性脑膜炎模型,造模组感染后每隔24 h分别腹腔、皮下注射生理盐水,CTRX组感染后每隔24 h给予头孢曲松皮下注射,CTRX+DEXA组感染后每隔24 h给予头孢曲松皮下注射联合地塞米松腹腔内注射。采用免疫组化、Western blot等方法检测各组脑组织occludin的表达。结果模型组脑组织occludin表达低于其他各组(P0.05),CTRX+DEXA组occludin表达高于CTRX组(P0.05)。结论细菌感染中枢神经系统后,occludin表达下调,地塞米松治疗能上调细菌性脑膜炎大鼠脑组织occludin蛋白的表达,此可能是早期应用地塞米松可保护中枢神经系统机制之一。
[Abstract]:Objective to observe the effect of dexamethasone on the expression of occlusal protein (occludin) in the brain of rats with bacterial meningitis. Methods Forty 3-week-old SD rats were randomly divided into three groups: model group, CTRX group and CTRX DEXA group. In CTRX group, ceftriaxone was injected subcutaneously with, CTRX DEXA every 24 hours after infection, and ceftriaxone combined with dexamethasone was injected intraperitoneally every 24 hours. Immunohistochemical, Western blot method was used to detect the expression of occludin in brain tissue of each group. Results the expression of occludin in brain tissue of model group was lower than that of other groups (P0.05 occludin expression in), CTRX DEXA group was higher than that in CTRX group (P0.05). Conclusion the expression of occludin is down-regulated after bacterial infection in the central nervous system. Dexamethasone treatment can up-regulate the expression of occludin protein in the brain of rats with bacterial meningitis, which may be one of the mechanisms of early application of dexamethasone to protect the central nervous system.
【作者单位】：郑州大学第三附属医院小儿神经内科;河南省红十字血液中心检验科;河南省医药科学研究院免疫研究室;
【基金】：河南省教育厅科学技术研究重点项目(编号:13A320662)
【分类号】：R515.2

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