无锡市艾滋病病毒感染分子流行病学调查

发布时间：2018-10-10 14:18

【摘要】：目的了解无锡市艾滋病病毒感染者和艾滋病患者人群中流行的HIV-1亚型及其分布特征。方法对无锡市HIV/AIDS人群进行流行病学个案调查,并扩增和测序env部分基因片段,根据env部分基因序列进行病毒亚型分析。结果有250例HIV/AIDS患者纳入研究,成功扩增并测序203例患者的env基因,共发现6种基因亚型,不同亚型及所占比例分别为流行重组型(circulating recombinant form,CRF)01_AE(58.6%,119/203)、CRF07_BC(20.7%,42/203)、CRF15_01B(9.4%,19/203)、CRF08_BC(8.4%,17/203)、B(2.0%,4/203)、C(1.0%,2/203),其中CRF01_AE在HIV-1/AIDS患者和男男同性性传播的感染人群中所占比例均最高,不同传播途径的病毒亚型分布差异有统计学意义(P0.001),系统进化分析显示不同传播途径间存在交叉感染的现象。结论目前无锡市流行的HIV-1基因型多样,CRF01_AE不仅是HIV/AIDS人群的优势流行毒株,更是男男同性性传播感染者的绝对优势毒株,不同传播途径间的交叉感染现象提示要加强对男男同性性行为人群的管理,密切监测HIV-1基因型及其变异趋势,以更有效地指导艾滋病防治工作。
[Abstract]:Objective to investigate the prevalence of HIV-1 subtypes and their distribution in HIV infected and AIDS patients in Wuxi. Methods Epidemiological investigation was carried out in Wuxi HIV/AIDS population, and partial env gene fragments were amplified and sequenced. Virus subtypes were analyzed according to partial env gene sequences. Results two hundred and fifty patients with HIV/AIDS were included in the study. The env gene of 203 patients was amplified and sequenced successfully. A total of 6 subtypes of env genes were found. Different subtypes and proportions are epidemic recombinant (circulating recombinant form,CRF) 01_AE (58.6%), CRF07_BC (20.7% 42 / 203), CRF15_01B (9.44% 19 / 203), CRF08_BC (8.4R 17 / 203), B (2.0% 20 / 203), C (1.0% 203), CRF01_AE is the highest percentage of people with HIV-1/AIDS and men sexually transmitted by men. There were significant differences in the distribution of virus subtypes among different transmission pathways (P0.001). Phylogenetic analysis showed that there was cross-infection among different transmission pathways. Conclusion HIV-1 genotypes are diverse in Wuxi City. CRF01_AE is not only the dominant epidemic strain in HIV/AIDS population, but also the absolute dominant strain of male sexually transmitted infections. The phenomenon of cross-infection among different transmission routes suggests that we should strengthen the management of men who have sex with men and closely monitor the genotype of HIV-1 and its variation trend in order to guide the prevention and treatment of AIDS more effectively.
【作者单位】：无锡市疾病预防控制中心疾病控制部;东南大学公共卫生学院流行病学与卫生统计学系;
【基金】：无锡市科技支撑计划(社会发展)项目(CSE01N1232)
【分类号】：R512.91

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