抗三磷酸甘油醛脱氢酶抗体IgG检测诊断日本血吸虫病的研究

发布时间：2018-10-11 20:09

【摘要】：目的探讨检测抗日本血吸虫三磷酸甘油酸脱氢酶(Sj GAPDH)抗体IgG诊断血吸虫病的价值。方法收集血吸虫现症感染患者治疗前、后不同时间点的血清及其他寄生虫病患者血清。以纯化的重组Sj GAPDH蛋白为抗原建立检测抗Sj GAPDH抗体IgG的常规酶联免疫吸附试验方法(Sj GAPDH ELISA)及生物素-亲和素信号放大酶联免疫吸附试验方法(Sj GAPDH BA ELISA),同时对收集的血清标本进行抗Sj GAPDH抗体IgG检测,并与检测抗SEA抗体IgG的SEA ELISA结果作比较,评价检测抗Sj GAPDH抗体IgG用于血吸虫病诊断与疗效考核的价值。结果 Sj GAPDH ELISA和Sj GAPDH BA ELISA检测79份现症感染血吸虫患者血清抗Sj GAPDH抗体IgG的敏感性分别为54.43%和91.14%,差异有统计学意义(P0.05%);特异性分别为93.85%和92.31%,差异无统计学意义(P0.05%)。Sj GAPDH ELISA检测患者在治疗后6个月血清特异性抗体阴转率为44.19%,治疗后12个月阴转率达53.49%。Sj GAPDH BA ELISA检测患者治疗后6个月血清特异性抗体阴转率为5.55%,治疗后12个月阴转率为9.72%。患者血清中抗SEA抗体IgG治疗后6和12个月均阳性。结论 Sj GAPDH蛋白在患者体内诱导短寿抗体反应,检测患者血清中的抗Sj GAPDH特异性抗体IgG水平及其在治疗前、后的变化具有一定的诊断与疗效考核价值。
[Abstract]:Objective to investigate the value of detecting (Sj GAPDH) antibody against triphosphate dehydrogenase (Sj GAPDH) in the diagnosis of schistosomiasis japonicum. Methods Serum samples of patients with schistosomiasis were collected at different time points before and after treatment. Using purified Recombinant Sj GAPDH protein as Antigen to establish routine Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Sj GAPDH IgG (Sj GAPDH ELISA) and (Sj GAPDH BA ELISA), for Biotin / Affinity signal Amplification Enzyme-linked Immunosorbent Assay To detect the anti Sj GAPDH antibody IgG. Compared with the SEA ELISA results of detecting anti SEA antibody IgG, the value of detecting anti Sj GAPDH antibody IgG for schistosomiasis diagnosis and curative effect evaluation was evaluated. Results the sensitivity of Sj GAPDH ELISA and Sj GAPDH BA ELISA in detecting anti-Sj GAPDH antibody IgG were 54.43% and 91.14%, respectively (P0.05%), the specificity was 93.85% and 92.31%, respectively (P0.05%). Sj GAPDH). The seroconversion rate of serum specific antibody was 44.19 in 6 months after ELISA treatment, 5.55 in 6 months after treatment and 9.72 in 12 months after treatment. The negative conversion rate of serum specific antibody was 5.55% at 12 months after treatment, 5.55% at 6 months after treatment and 9.72% at 12 months after treatment. Serum anti SEA antibody IgG was positive 6 and 12 months after treatment. Conclusion Sj GAPDH protein induces short life antibody reaction in patients, and the detection of IgG level of anti Sj GAPDH specific antibody in patients' serum and its changes before and after treatment have certain diagnostic and therapeutic value.
【作者单位】：江苏省寄生虫病分子生物学重点实验室卫生部寄生虫病预防与控制技术重点实验室江苏省血吸虫病防治研究所;江苏省淮安市淮安区疾病预防控制中心;
【基金】：国家自然科学基金项目(No.81201316,30972581) 国家传染病重大专项(No.2012ZX1004-220) 江苏省自然科学基金项目(No.BK2012544,BK20151120) 江苏省卫生厅血地寄科技项目(No.X201429,X201402)
【分类号】：R532.21

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