氯硝柳胺原位固化长效注射剂对感染日本血吸虫小鼠和水牛的预防效果
发布时间：2018-10-13 14:34
【摘要】：目的观察氯硝柳胺原位固化长效注射剂对感染日本血吸虫(Schistosoma japonicum)的小鼠和水牛的疗效。方法 50只昆明小鼠随机均分为2组,给药组小鼠皮下注射低浓度氯硝柳胺原位固化长效注射剂(71 mg/ml)450 mg/kg 1次,对照组小鼠不作任何处理。给药后第1、15、43、57和71天,各组分别取5只小鼠采用腹部贴片法感染日本血吸虫尾蚴(80±4)条/鼠。35 d后,处死小鼠,检获成虫,计算减虫率。10头水牛分成2组,给药组4头、对照组6头。给药组水牛皮下注射高浓度氯硝柳胺原位固化长效注射剂(222 mg/ml)30 mg/kg1次,对照组水牛不作任何处理。于给药后第1和3个月粪检,计算粪检阳性率,并观察水牛的不良反应。采用SPSS19.0软件进行统计学分析,组间比较采用t检验。结果给药后第1、15、43、57和71天感染日本血吸虫尾蚴,35 d后给药组小鼠的虫荷分别为(3.6±1.4)条、(17.5±8.6)条、(10.0±6.8)条、0条、(11.3±8.4)条,均少于对照组小鼠的虫荷(P0.05),且减虫率均超过50.0%。第1和3个月对水牛进行粪检,给药组均为阴性,对照组分别有2和3头阳性,其中1头牛给药后8 h出现精神沉郁。结论氯硝柳胺原位固化长效注射剂预防小鼠感染日本血吸虫尾蚴的作用达2个月,预防水牛感染日本血吸虫尾蚴的作用达3个月,具有现场应用预防血吸虫感染的潜力。
[Abstract]:Objective to observe the effect of niclosamide in situ solidified long-acting injection on mice and buffalo infected with Schistosoma japonicum (Schistosoma japonicum). Methods Fifty Kunming mice were randomly divided into two groups. The mice in the control group were given low concentration of niclosamide in situ solidified long-term injection (71 mg/ml) for 450 mg/kg subcutaneously, and the control group was not treated with any treatment. 5 mice were infected with cercariae of Schistosoma japonicum (80 卤4) per mouse by abdominal patch method in each group on the 1st day after administration. After 35 days, the mice were killed, the adult worms were seized, and the rate of worm reduction was calculated. 10 buffalo were divided into 2 groups and 4 in the administration group. There were 6 heads in the control group. High concentration of niclosamide in situ solidified long-acting injection (222 mg/ml) was injected subcutaneously for 30 mg/kg1 in the treatment group, and no treatment was given in the control group. The fecal positive rate was calculated at 1 and 3 months after administration, and the adverse reactions of buffalo were observed. SPSS19.0 software was used for statistical analysis and t test was used for comparison between groups. Results the Schistosoma japonicum cercariae were infected with Schistosoma japonicum cercariae on day 1, 15, 43, 57 and 71, respectively. After 35 days of administration, the worm load of mice in the administration group was (3.6 卤1.4), (17.5 卤8.6), (10.0 卤6.8), 0, (11.3 卤8.4), respectively, which was lower than that in the control group (P0.05), and the worm reduction rate was more than 50.0%. In the first and third months, the faecal examination of buffalo was negative in the administration group, and 2 and 3 positive in the control group. One of the buffaloes developed depression at 8 hours after administration. Conclusion niclosamide in situ solidified long-term injection can prevent mice from infection with Schistosoma japonicum cercariae for 2 months and buffalo for 3 months. It has the potential to prevent Schistosoma japonicum infection in the field.
【作者单位】： 中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合研究中心卫生部寄生虫病原与媒介生物学重点实验室;上海健康医学院;
【基金】：上海市公共卫生三年行动计划(No.GWIV-29,15GWZK0101) 上海市卫生和计划生育委员会科研课题(No.20154Y0051) 国家重点研发计划“重要热带病相关入侵媒介生物及其病原的动态分布与资源库建设(No.2016YFC1202000)”~~
【分类号】：R532.21
，
本文编号：2268967
[Abstract]:Objective to observe the effect of niclosamide in situ solidified long-acting injection on mice and buffalo infected with Schistosoma japonicum (Schistosoma japonicum). Methods Fifty Kunming mice were randomly divided into two groups. The mice in the control group were given low concentration of niclosamide in situ solidified long-term injection (71 mg/ml) for 450 mg/kg subcutaneously, and the control group was not treated with any treatment. 5 mice were infected with cercariae of Schistosoma japonicum (80 卤4) per mouse by abdominal patch method in each group on the 1st day after administration. After 35 days, the mice were killed, the adult worms were seized, and the rate of worm reduction was calculated. 10 buffalo were divided into 2 groups and 4 in the administration group. There were 6 heads in the control group. High concentration of niclosamide in situ solidified long-acting injection (222 mg/ml) was injected subcutaneously for 30 mg/kg1 in the treatment group, and no treatment was given in the control group. The fecal positive rate was calculated at 1 and 3 months after administration, and the adverse reactions of buffalo were observed. SPSS19.0 software was used for statistical analysis and t test was used for comparison between groups. Results the Schistosoma japonicum cercariae were infected with Schistosoma japonicum cercariae on day 1, 15, 43, 57 and 71, respectively. After 35 days of administration, the worm load of mice in the administration group was (3.6 卤1.4), (17.5 卤8.6), (10.0 卤6.8), 0, (11.3 卤8.4), respectively, which was lower than that in the control group (P0.05), and the worm reduction rate was more than 50.0%. In the first and third months, the faecal examination of buffalo was negative in the administration group, and 2 and 3 positive in the control group. One of the buffaloes developed depression at 8 hours after administration. Conclusion niclosamide in situ solidified long-term injection can prevent mice from infection with Schistosoma japonicum cercariae for 2 months and buffalo for 3 months. It has the potential to prevent Schistosoma japonicum infection in the field.
【作者单位】： 中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合研究中心卫生部寄生虫病原与媒介生物学重点实验室;上海健康医学院;
【基金】：上海市公共卫生三年行动计划(No.GWIV-29,15GWZK0101) 上海市卫生和计划生育委员会科研课题(No.20154Y0051) 国家重点研发计划“重要热带病相关入侵媒介生物及其病原的动态分布与资源库建设(No.2016YFC1202000)”~~
【分类号】：R532.21
，
本文编号：2268967
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