HIV相关型KS患者组织中TLR4的表达情况及意义
发布时间：2018-10-13 17:28
【摘要】：目的：探讨Toll样受体4基因在HIV相关型KS (Kaposi's sarcoma)肿瘤组织和及正常组织中的表达。方法：收集2011年5月至2013年7月在新疆医科大学第一附属医院及新疆传染病医院就诊并经病理明确诊断的患者KS肿瘤组织35例,包括HIV-KS26例,经典KS9例,应用实时荧光定量PCR技术、免疫组化方法检测TLR4在卡波西肉瘤组织中的表达与分布。应用Western Blot方法检测TLR4信号通路上蛋白的表达。结果：TLR4基因在KS肿瘤组织中mRNA水平的表达均低于正常组织,差异有统计学意义(P=0.0017),经典KS及正常对照之间表达有显著性差异(P=0.0497), HIV-KS与正常对照之间表达也有显著性差异(P=0.0054)。而在经典KS及HIV-KS之间表达无统计学意义(P=0.46)。免疫组化中,在经典KS及HIV-KS病损皮肤组织真皮层可见TLR4阳性细胞,主要表达于内皮细胞和异形细胞胞浆和胞膜。蛋白水平：HIV-KS及经典KS组织中可见到Ras、Raf、ERK、IκB-α、P50、P65表达较正常组织高,P0.05,且有显著性差异,而在]HIV-KS和经典KS之间无显著性差异。其余蛋白在正常及KS之间表达无显著性差异。结论：在艾滋病患者中,TLR4低表达与HIV-KS的发生密切相关。
[Abstract]:Aim: to investigate the expression of Toll-like receptor 4 gene in HIV-associated KS (Kaposi's sarcoma) tumors and normal tissues. Methods: from May 2011 to July 2013, 35 patients with KS tumor tissue, including HIV-KS26, classic KS9, were collected from the first affiliated Hospital of Xinjiang Medical University and Xinjiang Infectious Diseases Hospital. The expression and distribution of TLR4 in Kaposi's sarcoma were detected by real-time fluorescence quantitative PCR and immunohistochemistry. Western Blot method was used to detect the expression of protein in TLR4 signaling pathway. Results: the expression of TLR4 gene in KS was significantly lower than that in normal tissue (P0. 0017). There was a significant difference between classical KS and normal control (P0. 0497). There was also a significant difference between HIV-KS and normal control (P0. 0054). However, there was no significant difference between the expression of KS and HIV-KS (P < 0. 46). In immunohistochemistry, TLR4 positive cells were found in the dermis of classical KS and HIV-KS lesions, mainly expressed in the cytoplasm and membrane of endothelial cells and allogeneic cells. Protein level: the expression of Ras,Raf,ERK,I 魏 B- 伪 and P50 p65 in HIV-KS and classical KS tissues was higher than that in normal tissues, and there was significant difference between HIV-KS and classical KS, but there was no significant difference between HIV-KS and classical KS. There was no significant difference in the expression of other proteins between normal and KS. Conclusion: the low expression of TLR4 is closely related to the occurrence of HIV-KS in AIDS patients.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.91
本文编号：2269388
[Abstract]:Aim: to investigate the expression of Toll-like receptor 4 gene in HIV-associated KS (Kaposi's sarcoma) tumors and normal tissues. Methods: from May 2011 to July 2013, 35 patients with KS tumor tissue, including HIV-KS26, classic KS9, were collected from the first affiliated Hospital of Xinjiang Medical University and Xinjiang Infectious Diseases Hospital. The expression and distribution of TLR4 in Kaposi's sarcoma were detected by real-time fluorescence quantitative PCR and immunohistochemistry. Western Blot method was used to detect the expression of protein in TLR4 signaling pathway. Results: the expression of TLR4 gene in KS was significantly lower than that in normal tissue (P0. 0017). There was a significant difference between classical KS and normal control (P0. 0497). There was also a significant difference between HIV-KS and normal control (P0. 0054). However, there was no significant difference between the expression of KS and HIV-KS (P < 0. 46). In immunohistochemistry, TLR4 positive cells were found in the dermis of classical KS and HIV-KS lesions, mainly expressed in the cytoplasm and membrane of endothelial cells and allogeneic cells. Protein level: the expression of Ras,Raf,ERK,I 魏 B- 伪 and P50 p65 in HIV-KS and classical KS tissues was higher than that in normal tissues, and there was significant difference between HIV-KS and classical KS, but there was no significant difference between HIV-KS and classical KS. There was no significant difference in the expression of other proteins between normal and KS. Conclusion: the low expression of TLR4 is closely related to the occurrence of HIV-KS in AIDS patients.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.91
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