细胞因子IL-4、IL-9和IgE在肠道蠕虫感染者中的水平及临床意义

发布时间：2018-10-14 13:45

【摘要】：目的检测肠道蠕虫感染者外周血中细胞因子白细胞介素-4(IL-4)、IL-9和Ig E的水平,并分析其与临床症状和虫种之间的相关性。方法收集2010年1月至2014年7月河南省人民医院儿科收治的肠道蠕虫感染者55例,包括蛔虫感染者18例(32.7%)、钩虫感染者8例(14.5%)、鞭虫感染者7例(12.7%)和蛲虫感染者22例(40.0%)。ELISA检测该55例肠道蠕虫感染者治疗前后以及健康对照组外周血中IL-4、IL-9和Ig E水平,并分析其与临床症状和虫种之间的相关性。结果肠道蠕虫感染组药物治疗前外周血中IL-4、IL-9和Ig E水平分别为(157.42±41.0)pg/ml、(59.9±21.7)pg/ml和(316.6±129.0)IU/ml,均高于健康对照组的(39.0±23.5)pg/ml、(21.3±12.5)pg/ml和(127.7±117.6)IU/ml(P0.01);治疗后外周血中IL-4、IL-9和Ig E水平显著降低,分别为(98.1±41.7)pg/ml、(38.7±14.1)pg/ml和(253.1±94.0)IU/ml,但仍高于健康对照组(P0.05)。55例患者中,消化道出血者IL-9水平为(76.1±23.5)pg/ml,明显高于腹部不适和排便习惯改变者(54.3±22.1)pg/ml(P0.05),但低于并发过敏性皮炎者(108.5±33.4)pg/ml(P0.05),3组不同症状患者Ig E和IL-4水平差异无统计学意义(P0.05)。此外,蛲虫、蛔虫、钩虫和鞭虫感染组IL-9水平分别为(120.3±41.0)pg/ml、(90.1±29.7)pg/ml、(77.3±18.3)pg/ml和(62.5±24.3)pg/ml,蛲虫感染组高于蛔虫、钩虫和鞭虫感染组(P0.01),各虫种感染组IL-4和Ig E水平差异无统计学意义(P0.05)。蛔虫和钩虫感染者IL-9的水平差异无统计学意义(P0.05),但均高于鞭虫感染者(P0.01),3者之间IL-4和Ig E水平差异无统计学意义(P0.05)。结论 IL-4、IL-9和Ig E水平与肠道蠕虫感染明显相关,IL-9水平因感染虫种和临床症状不同存在较大的差异。
[Abstract]:Objective to detect the levels of cytokines interleukin-4 (IL-4), IL-9 and Ig E in peripheral blood of intestinal worm infected patients, and to analyze their correlation with clinical symptoms and insect species. Methods from January 2010 to July 2014, 55 cases of intestinal helminth infection in pediatrics department of Henan Provincial people's Hospital were collected. The levels of IL-4,IL-9 and Ig E in peripheral blood of 55 cases of intestinal helminth infection before and after treatment were detected by). ELISA, including 18 cases (32.7%) of roundworm infection, 8 cases (14.5%) of hookworm infection, 7 cases (12.7%) of Trichuris trichuris infection and 22 cases (40.0%) of Enterobius vermicularis infection. The correlation between clinical symptoms and insect species was analyzed. Results the levels of IL-4,IL-9 and Ig E in patients with intestinal helminth infection were (157.42 卤41.0) pg/ml, (59.9 卤21.7) pg/ml and (316.6 卤129.0) IU/ml, respectively, higher than those in healthy controls (39.0 卤23.5) pg/ml, (21.3 卤12.5) pg/ml and (127.7 卤117.6) IU/ml (P0.01), and the levels of IL-4,IL-9 and Ig E in peripheral blood were significantly decreased after treatment. (98. 1 卤41. 7) pg/ml, (38. 7 卤14. 1) pg/ml and (253.1 卤94. 0) IU/ml, but still higher than those in the healthy control group (P0.05). The IL-9 level in patients with gastrointestinal hemorrhage was (76.1 卤23.5) pg/ml, significantly higher than that in patients with abdominal discomfort and defecation change (54.3 卤22.1) pg/ml (P0.05), but lower than that in patients with allergic dermatitis (108.5 卤33.4) pg/ml (P0.05). There was no significant difference in Ig E and IL-4 levels among the three groups with different symptoms (P0.05). In addition, the IL-9 levels of Enterobius vermicularis, Ascaris lumbricoides, hookworm and Trichuris infection groups were (120.3 卤41.0) pg/ml, (90.1 卤29.7) pg/ml and (62.5 卤24.3) pg/ml, Enterobius vermicularis infection, respectively. There was no significant difference in IL-4 and Ig E levels among the infected groups (P0.05). The levels of IL-9 in Ascaris lumbricoides and hookworm infection were not significantly different (P0.05), but were higher than those in Trichuris infection (P0.01). There was no significant difference in IL-4 and Ig E levels among three individuals (P0.05). Conclusion the levels of IL-4,IL-9 and Ig E were significantly correlated with intestinal helminth infection, and the IL-9 levels were significantly different among different infection species and clinical symptoms.
【作者单位】：河南省人民医院检验科;河南省疾病预防控制中心;郑州市儿童医院;
【基金】：河南省医学科技攻关计划项目(No.201304045)~~
【分类号】：R532

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