Dectin-1在热处理光滑念珠菌刺激大鼠气道上皮细胞中的免疫调节作用

发布时间：2018-10-22 17:36

【摘要】：目的:探讨Dectin-1在热处理光滑念珠菌刺激大鼠气道上皮细胞(RTECs)中的作用及对IL-10和TNF-α表达的影响。方法:将体外培养RTECs随机分为三组:对照组加无菌生理盐水;真菌刺激组加热处理光滑念珠菌孵育;抑制剂干预组在昆布多糖预处理后加热处理光滑念珠菌孵育。分别在孵育0h、2h、4h、6h后终止,收集细胞及上清液。在光镜下观察细胞形态及生长情况,用MTT法检测细胞存活率,Western blot法检测Dectin-1蛋白的表达,ELISA法检测IL-10和TNF-α的表达。结果:热处理光滑念珠菌抑制RTECs的增殖;真菌刺激组、抑制剂干预组2h、4h、6h细胞Dectin-1、IL-10、TNF-α的表达均高于对照组(P均0.05)。在抑制剂干预组,同时间点Dectin-1、IL-10、TNF-α的表达均低于真菌刺激组(P0.05)。结论:Dectin-1是RTECs识别热处理光滑念珠菌的重要受体,并诱导其释放IL-10和TNF-α,介导炎症反应的发生。Dectin-1抑制剂昆布多糖能抑制IL-10和TNF-α的表达,加重机体损伤。
[Abstract]:Aim: to investigate the effect of Dectin-1 on the expression of IL-10 and TNF- 伪 in cultured rat airway epithelial cells stimulated by Candida smoothing. Methods: RTECs cultured in vitro was randomly divided into three groups: control group with aseptic saline; fungal stimulation group with heating treated Candida smooth; inhibitor intervention group with heat treated Candida smoothing after pretreatment with laminin polysaccharide. The cells and supernatant were collected after incubation for 2 h and 4 h respectively for 6 h. The morphology and growth of cells were observed under light microscope. The expression of Dectin-1 protein was detected by MTT method, and the expression of IL-10 and TNF- 伪 was detected by ELISA method. Results: heat treated Candida smoothing inhibited the proliferation of RTECs, and the expression of Dectin-1,IL-10,TNF- 伪 in fungal stimulation group and inhibitor intervention group was significantly higher than that in control group (P 0.05). In the inhibitor intervention group, the expression of Dectin-1,IL-10,TNF- 伪 at the same time was lower than that in the fungal stimulation group (P0.05). Conclusion: Dectin-1 is an important receptor for RTECs to recognize heat treated Candida albicans, and to induce the release of IL-10 and TNF- 伪, thus mediating inflammation. Dectin-1 inhibitor laminin polysaccharide can inhibit the expression of IL-10 and TNF- 伪 and aggravate the damage of organism.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R519

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