乙型肝炎组织iNOS的表达与炎症分级的相关性研究

发布时间：2018-10-31 21:02

【摘要】：目的 :探讨诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在乙型肝炎组织中的表达情况及与炎症分级的相关性。方法:收集HBs Ag阳性肝穿刺及手术标本71例,按肝炎修正组织学活性指数(histology activity index,HAI)记分法分为G0、G1、G2、G3、G45组,运用免疫组织化学法检测iNOS在各组中的表达情况,并分析其与炎症分级的相关性。结果:iNOS阳性染色主要定位于肝细胞的细胞浆,部分淋巴细胞胞浆中也可见iNOS阳性染色;随着炎症程度增加,iNOS在肝细胞和淋巴细胞中的表达强度均逐渐增强,且表达强度与炎症分级呈正相关(肝细胞r=0.5148,P=0.0000;淋巴细胞r=0.3491,P=0.0016);肝细胞中iNOS的表达与淋巴细胞iNOS的表达存在正相关关系(r=0.4762,P=0.0000)。结论:iNOS可作为乙型肝炎炎症分级的一项参考指标,可能与乙型肝炎病理生理过程密切相关。
[Abstract]:Aim: to investigate the expression of inducible nitric oxide synthase (inducible nitric oxide synthase,iNOS) in hepatitis B tissues and its correlation with inflammatory grade. Methods: 71 cases of HBs Ag positive liver puncture and operation were collected. According to the modified histological activity index (histology activity index,HAI), the patients were divided into two groups: group G _ (0) and G _ (1) G _ (2) G _ (3) G _ (45). The expression of iNOS in each group was detected by immunohistochemical method. The correlation between inflammatory grade and inflammatory grade was analyzed. Results: iNOS positive staining was mainly located in the cytoplasm of hepatocytes, and iNOS positive staining could also be seen in some lymphocytes cytoplasm. With the increase of the degree of inflammation, the expression of iNOS in hepatocytes and lymphocytes increased gradually, and the expression intensity was positively correlated with the inflammatory grade (hepatocyte rn 0.5148 PnP 0.0000, lymphocyte rn 0.3491 P0. 0016). There was a positive correlation between the expression of iNOS and the expression of iNOS in lymphocytes (r 0.4762 P0. 0000). Conclusion: iNOS may be a reference index for hepatitis B inflammation grade and may be closely related to the pathophysiological process of hepatitis B.
【作者单位】：南通市第三人民医院病理科;
【基金】：南通市前沿与关键技术创新项目(MS22015087) 南通市卫计委青年科研基金资助项目(WQ2015034、WQ2016016)
【分类号】：R512.62

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