PRU株弓形虫感染小鼠后脑组织免疫病理的研究

发布时间：2018-11-02 15:34

【摘要】：目的了解弓形虫PRU株感染小鼠后脑内局部细胞因子对脑组织的免疫病理作用。方法 51只ICR小鼠分为感染组(33只)和对照组(18只),感染组经腹腔注射弓形虫PRU株10个包囊/鼠,对照组腹腔注射同等量的PBS。于感染后第5d、10d、15d、20d、30d和90d,剖杀感染组小鼠5只和对照组小鼠3只,取脑组织,部分作病理切片;部分提取其RNA,检测IFN-γ、IL-4、IL-6和TNF-αmRNA;部分获取脑组织上清液,用ELISA法测定上述细胞因子。结果相对于正常对照组,感染小鼠脑组织中IFN-γ于感染后第5d开始升高,第10d时下降达到最低,之后开始上升;TNF-α在感染第10d或15d时明显降低,接着上升并于第30d时达到高峰;IL-6在感染第5d时开始升高,感染第10d时降至最低,之后缓慢上升,并于第30d后达到高峰;IL-4未见明显变化。结论弓形虫PRU感染ICR小鼠的过程中,脑组织中IFN-γ、IL-6和TNF-α细胞因子在感染第10d或15d的低表达有利于弓形虫逃避宿主的免疫杀伤作用,导致速殖子在脑组织中存活;同时细胞因子的分泌不平衡,导致小鼠脑组织中出现弓形虫包囊与病理变化共存的隐性感染状态。
[Abstract]:Objective to investigate the immunopathologic effects of local cytokines in the brain of mice infected with Toxoplasma PRU strain. Methods 51 ICR mice were divided into infection group (n = 33) and control group (n = 18). The infected group received intraperitoneal injection of Toxoplasma gondii PRU strain 10 cysts per mouse, and the control group received the same amount of PBS. intraperitoneally. At the 5th day, 10 days after infection, 15 days after infection, 20 days after infection, 30 and 90 days after infection, 5 mice in the infected group and 3 mice in the control group were dissected, brain tissues were taken for pathological sections, and IFN- 纬, IL-4,IL-6 and TNF- 伪 mRNA; were partly extracted for detection of IFN- 纬, IL-4,IL-6 and TNF- 伪 mRNA;. The supernatant of brain tissue was obtained and the cytokines were determined by ELISA method. Results compared with the normal control group, the IFN- 纬 in the brain tissue of the infected mice began to increase at the 5th day after infection, reached the lowest level on the 10th day, and then began to rise. TNF- 伪 decreased significantly on the 10th or 15th day of infection, then increased and reached the peak on the 30th day, IL-6 began to increase on the 5th day, reached the lowest level on the 10th day, then increased slowly, and reached the peak at the 30th day after infection. There was no obvious change in IL-4. Conclusion during ICR mice infected with Toxoplasma gondii PRU, the low expression of IFN- 纬, IL-6 and TNF- 伪 cytokines in brain tissue on the 10th or 15th day of Toxoplasma gondii infection is beneficial to the immune killing effect of Toxoplasma gondii on the 10th or 15th day of infection, leading to the survival of tachyzoites in brain tissue. At the same time, the secretion of cytokines was unbalanced, resulting in the occult infection of Toxoplasma gondii cyst and pathological changes in the brain tissue of mice.
【作者单位】： 贵州省疾病预防控制中心;贵阳医学院寄生虫教研室;
【分类号】：R531.8

【相似文献】

相关期刊论文 前10条

1 张永源;郝连杰;;病毒性肝炎的免疫病理[J];临床内科杂志;1985年03期

2 吴先林;;皮肤免疫病理(一)[J];皮肤病防治;1985年Z1期

3 敬业;第四届全国免疫病理学术会议召开[J];中国免疫学杂志;1988年04期

4 孙晓明;叶瑞云;;急性出血性坏死性肠炎——从免疫病理探讨其发病机制的进展[J];临床医学;1986年04期

5 杨培增;第五届国际眼免疫和免疫病理会议概况[J];国外医学.眼科学分册;1991年06期

6 马世英;;中国病理生理学会第六届免疫病理专业学术会暨中国抗癌协会肿瘤标志专业学术会简介[J];沈阳部队医药;1994年03期

7 陈凌,郭若冰;免疫病理与原发性扩张型心肌病[J];心血管病学进展;1999年01期

8 付文静;郭光红;付爱峰;张建明;张静;;女性生殖道沙眼衣原体感染免疫病理机制研究现状[J];中国皮肤性病学杂志;2010年11期

9 陈素群;黄毅;王小林;;心脏疾病的免疫病理机制研究进展[J];中国医药指南;2013年07期

10 鄢璞，苏燕莉，陈光华，张美德，黄炜，曾荣新，郑秀霞，，赵西平，郝连杰;小儿乙型肝炎血清、病理及免疫病理分析[J];临床肝胆病杂志;1996年01期

相关会议论文 前3条

1 王金泉;刘志红;胡伟新;陈惠萍;曾彩虹;唐政;黎磊石;;致密物沉积病的初步临床观察[A];中华医学会肾脏病学分会2006年学术年会论文集[C];2006年

2 闵迅;陈代雄;王永伦;方宁;万卫红;刘金伟;肖瑜;周振忠;;NF-κB在Graves病免疫病理机制中的意义[A];中华医学会第七次全国检验医学学术会议资料汇编[C];2008年

3 张迪展;王广进;亓兴亮;杜东红;;免疫病理支持增殖型天疱疮[A];2009全国中西医结合皮肤性病学术会议论文汇编[C];2009年

相关重要报纸文章 前1条

1 陶春祥;NO的抗炎作用研究[N];中国医药报;2004年

相关硕士学位论文 前2条

1 张凤玲;系膜增生性肾小球肾炎气阴两虚证为主者与免疫病理关系的研究[D];福建中医药大学;2010年

2 马钰;OX40/OX40L分子在B6.MRL-Faslpr/J狼疮小鼠免疫病理中的作用机制[D];苏州大学;2013年

本文编号：2306201