表达结核分枝杆菌融合抗原TB10.4-HspX的重组酵母免疫效果研究
发布时间：2018-11-02 18:08
【摘要】：结核病是一种严重危害人类健康的传染病,其防治难点主要在于抗药性菌株的出现以及唯一的抗结核疫苗——卡介苗的效果不理想.为发展新型抗结核疫苗,本研究中构建了表达结核分枝杆菌融合抗原TB10.4-HspX(TB10H)的重组酿酒酵母,分别以皮下注射和滴鼻两种方式免疫小鼠,检测免疫效果.结果显示,该重组酿酒酵母通过皮下注射的方式免疫后刺激小鼠产生TB10H特异性抗体IgG,分泌Th1型细胞因子IFN-γ,而且重组酵母免疫后在一定程度上刺激小鼠树突状细胞的成熟分化和抗原递呈作用.一系列结果表明,表达融合抗原TB10H的重组酿酒酵母能够有效激发小鼠抗原特异性免疫应答.
[Abstract]:Tuberculosis (TB) is a serious infectious disease which is harmful to human health. The prevention and treatment difficulties of TB mainly lie in the emergence of drug-resistant strains and the unsatisfactory effect of the only anti-TB vaccine, BCG (Bacillus Calmette Guerin) vaccine. In order to develop a novel anti-tuberculosis vaccine, recombinant Saccharomyces cerevisiae expressing mycobacterium tuberculosis fusion antigen TB10.4-HspX (TB10H) was constructed in this study. Mice were immunized with subcutaneous injection and nasal drip respectively. The results showed that the recombinant Saccharomyces cerevisiae was immunized by subcutaneous injection to stimulate the production of TB10H specific antibody IgG, to secrete Th1 type cytokine IFN- 纬. Moreover, the recombinant yeast stimulated the maturation, differentiation and antigen presentation of mouse dendritic cells to some extent. A series of results showed that recombinant Saccharomyces cerevisiae expressing fusion antigen TB10H could effectively stimulate the antigen-specific immune response of mice.
【作者单位】： 复旦大学生命科学学院遗传学研究所;复旦大学遗传工程国家重点实验室;
【基金】：“十二五”国家科技重大专项(2012ZX10003008) 上海市基础研究重点项目(08JC1401100)
【分类号】：R52
[Abstract]:Tuberculosis (TB) is a serious infectious disease which is harmful to human health. The prevention and treatment difficulties of TB mainly lie in the emergence of drug-resistant strains and the unsatisfactory effect of the only anti-TB vaccine, BCG (Bacillus Calmette Guerin) vaccine. In order to develop a novel anti-tuberculosis vaccine, recombinant Saccharomyces cerevisiae expressing mycobacterium tuberculosis fusion antigen TB10.4-HspX (TB10H) was constructed in this study. Mice were immunized with subcutaneous injection and nasal drip respectively. The results showed that the recombinant Saccharomyces cerevisiae was immunized by subcutaneous injection to stimulate the production of TB10H specific antibody IgG, to secrete Th1 type cytokine IFN- 纬. Moreover, the recombinant yeast stimulated the maturation, differentiation and antigen presentation of mouse dendritic cells to some extent. A series of results showed that recombinant Saccharomyces cerevisiae expressing fusion antigen TB10H could effectively stimulate the antigen-specific immune response of mice.
【作者单位】： 复旦大学生命科学学院遗传学研究所;复旦大学遗传工程国家重点实验室;
【基金】：“十二五”国家科技重大专项(2012ZX10003008) 上海市基础研究重点项目(08JC1401100)
【分类号】：R52
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