细胞色素P450 1A2和2C19基因多态性与抗结核药物性肝损伤的关系

发布时间：2018-11-08 08:02

【摘要】：目的探讨细胞色素P450 1A2和2C19基因多态性与抗结核药物性肝损伤的相关性。方法采用1∶1病例对照研究,以抗结核药治疗致肝损伤的结核病患者为病例组,无肝损伤者为对照组,共179对。采用聚合酶链反应限制性片段长度多态方法检测CYP1A2基因734C/A和CYP2C19基因681G/A位点基因型。采用SPSS17.0条件Logistic回归进行数据分析。结果CYP1A2基因734C/A位点C、A等位基因频率在病例组和对照组频率分别为37.2%、62.8%、和47.2%、52.8%;CYP2C19基因的681G/A位点G、A等位基因频率在病例组和对照组频率分别为59.8%、40.2%和62.8%、37.2%,CYP1A2基因734C/A位点多态性与抗结核药物性肝损伤的发生有关(P0.05),CA、AA基因型的比值比(odds ratio,OR)分别为1.951和1.916,而尚不能认为CYP2C19基因681G/A位点多态性与抗结核药物性肝损伤发生有关。结论 CYP1A2基因734C/A位点多态性与抗结核药物性肝损伤的发生有关,并且携带CA、AA突变基因型的个体是危险人群。
[Abstract]:Objective to investigate the relationship between cytochrome P450 1A2 and 2C19 gene polymorphisms and antituberculotic liver injury. Methods A 1:1 case-control study was carried out in which 179 pairs of tuberculosis patients with liver injury were treated with antituberculotics and those without liver injury. Polymerase chain reaction restriction fragment length polymorphism was used to detect the genotypes of CYP1A2 734C/A and CYP2C19 681G/A loci. The data were analyzed by SPSS17.0 conditional Logistic regression. Results the frequency of CMA A allele at 734C/A locus of CYP1A2 gene was 37.22.8in the case group and 52.8g in the control group, respectively. The frequency of 681G/A locus GMA A allele of CYP2C19 gene was 59.2% and 62.8% in the case group and control group, respectively. The 734C/A locus polymorphism of CYP1A2 gene was associated with the occurrence of anti-tuberculosis drug induced liver injury (P0.05). The ratio of CA,AA genotype (odds ratio,OR) was 1.951 and 1.916, respectively. However, we can not conclude that the polymorphism of 681G/A locus of CYP2C19 gene is related to the occurrence of liver injury induced by antituberculous drugs. Conclusion the polymorphism of 734C/A locus of CYP1A2 gene is associated with the occurrence of liver injury induced by antituberculous drugs, and individuals with CA,AA mutation genotype are at risk.
【作者单位】：河北联合大学公共卫生学院河北省煤矿卫生与安全重点实验室;唐山市结核病医院检验科;
【基金】：国家自然科学基金资助项目(81041096) 唐山市重点实验室资助项目(08150201A-1-8)
【分类号】：R575;R52

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