阴道及宫颈尖锐湿疣组织中HPV感染基因型的研究

发布时间：2018-11-11 08:48

【摘要】：目的探讨阴道和宫颈尖锐湿疣(CA)组织中人乳头瘤病毒(HPV)感染基因型分布情况及其临床意义。方法采用PCR和基因芯片技术对56例阴道CA组织和62例宫颈CA组织标本进行23种HPV基因分型检测,并对其患者进行临床病理资料分析。结果 56例阴道CA组织中检出HPV阳性感染51例,感染率为91.07%;其中一重HPV感染32例,感染率为57.14%;多重HPV感染19例,感染率为33.93%;一重感染中HPV11型19例,HPV6型11例;多重HPV感染中HPV6+11型4例,HPV11+52型3例。62例宫颈CA组织中检出HPV阳性感染59例,感染率为95.16%;其中一重HPV感染45例,感染率为72.58%;多重HPV感染14例,感染率为22.58%;一重感染中HPV11型29例,HPV6型14例;多重HPV感染中HPV6+11型4例,HPV11+18型2例。结论 HPV6、11、6+11、11+52型是阴道CA的主要致病类型,HPV6、11、6+11、11+18型是宫颈CA的主要致病类型。PCR结合基因芯片技术是一种比较适合临床进行HPV分型诊断的方法,其敏感性高,特异性好,对我国女性阴道和宫颈CA及其癌瘤的防治以及疫苗的研究具有十分重要的意义。
[Abstract]:Objective to investigate the genotype distribution and clinical significance of human papillomavirus (HPV) (HPV) infection in (CA) tissues of vaginal and cervical condyloma acuminatum. Methods 23 HPV genotypes were detected by PCR and gene chip technique in 56 cases of vaginal CA and 62 cases of cervical CA, and the clinicopathological data of the patients were analyzed. Results 51 cases of HPV positive infection were detected in 56 cases of vaginal CA, including 32 cases of primary HPV infection (57.14%), 19 cases of multiple HPV infection (33.93%), 32 cases of primary HPV infection (57.14%), 19 cases of multiple HPV infection (33.93%). 19 cases of HPV11 type, 11 cases of HPV6 type, 4 cases of HPV6 11 type and 3 cases of HPV11 52 type in multiple HPV infection, 59 cases of HPV positive infection were detected in 62 cases of cervical CA, the infection rate was 95.16%. There were 45 cases of primary HPV infection (72.58), 14 cases of multiple HPV infection (22.58%), 29 cases of HPV11 type and 14 cases of HPV6 type in primary infection, 4 cases of HPV6 11 type and 2 cases of HPV11 18 type in multiple HPV infection. Conclusion HPV6,11,6 111152 is the main pathogenic type of CA in vagina, and HPV6,11,6 1111 11 18 is the main pathogenic type of cervical CA. PCR combined with gene chip technique is a more suitable method for clinical diagnosis of HPV typing with high sensitivity and good specificity. It is of great significance for the prevention and treatment of vaginal and cervical CA and its carcinomas and the study of vaccines in Chinese women's vagina and cervix.
【作者单位】：常熟市中医院常熟市新区医院;盐城市第一人民医院病理科;南京中医药大学第三附属医院病理科江苏省HPV协作组;
【基金】：江苏省南京市卫生局中医专项支助项目(2009-92)
【分类号】：R752.53

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