TNF-α基因多态性与青海地区藏族人群乙肝病毒易感性的相关性研究

发布时间：2018-11-17 14:52

【摘要】：目的：分析青海地区藏族人群中TNF-α基因的2个位点-238及-308与慢性乙型肝炎易感性之间的关系，并筛选出相关的主要位点，为青海地区藏族人群的HBV易感性风险的预测奠定分子学基础。从而为临床诊断及预防慢性乙型肝炎提供新的思路和方法。方法： (1)研究对象：以250例青海地区藏族慢性乙肝患者(病例组)及134例健康者(对照组)做为研究对象。 (2)基因多态性分析：采用血液基因组小量提取试剂盒提取外周血DNA，运用飞行质谱技术分析测定基因位点TNF-α的-238A/G、-238A/A、-238G/G、-308G/A、-308G/G的基因型。 (3)数据分析：计算各位点基因型及等位基因频率，比较组间各基因型的差异，，并进行Hardy-Weinberg平衡吻合度检验，应用logistic多元回归分析各基因型与乙肝易感的相关性。结果： (1)-238位点在病例组以及对照组之间的频率分布:-238A/G在病例组和对照组分别为8.4%和8.2%，经统计学分析结果表明,各组间的分布频率未见显著性差异；-238A/A在病例组和对照组分别为0.4%和0.0%，经统计学分析结果表明,各组间的分布频率未见显著性差异；-238G/G在病例组和对照组分别为91.2%和91.8%，经统计学分析结果表明,各组间的分布频率未见显著性差异； (2)-308在各临床类型中的分布：-308G/A在慢性病毒性乙型肝炎以及正常对照组分别为7.6%和7.5%,各组间的分布频率比较亦未见显著性统计学差异；-308G/G在各组分别为92.4%和92.5%,各组间的分布频率比较亦未见显著性统计学差异；结论： (1)TNF-α-238基因位点多态性与青海地区藏族人群HBV感染的易感性可能无关； (2)TNF-α-308基因位点多态性与青海地区藏族人群HBV感染的易感性可能无关。
[Abstract]:Objective: to analyze the relationship between the susceptibility of chronic hepatitis B (CHB) and the two loci of TNF- 伪 gene-238 and -308 in Tibetan population in Qinghai area, and to screen out the main related loci. To lay a molecular foundation for the prediction of HBV susceptibility risk in Qinghai Tibetan population. So as to provide new ideas and methods for clinical diagnosis and prevention of chronic hepatitis B. Methods: (1) study subjects: 250 Tibetan chronic hepatitis B patients (case group) and 134 healthy persons (control group) in Qinghai. (2) Polymorphism Analysis of Gene: peripheral DNA, was extracted from peripheral blood by using a small quantity extraction kit of blood genome. The TNF- 伪 gene locus was analyzed by FMS to determine -238A / G / -238A / An / 238G / A / G / 308G / A, -genotype of 308G/G. (3) data analysis: the frequencies of genotypes and alleles were calculated, the differences of genotypes were compared, and the Hardy-Weinberg equilibrium coincidence test was performed. The correlation between genotypes and susceptibility to hepatitis B was analyzed by logistic multivariate regression analysis. Results: (1) the frequency distribution of -238 locus in the case group and the control group:-238A/G was 8.4% and 8.2% in the case group and the control group, respectively. There was no significant difference in distribution frequency among groups. -238A/A in the case group and the control group were 0.4% and 0.0.The results of statistical analysis showed that there was no significant difference in the distribution frequency among the groups. -238G/G was 91.2% and 91.8% in the case group and the control group, respectively. The results of statistical analysis showed that there was no significant difference in the distribution frequency among the three groups. (2) the distribution of -308 in each clinical type:-308G/A in chronic toxic hepatitis B and normal control group were 7.6% and 7.5%, respectively. -308G/G was 92.4% and 92.5% in each group, and there was no significant statistical difference in the distribution frequency between each group. Conclusion: (1) the polymorphism of TNF- 伪 -238 gene may not be associated with the susceptibility to HBV infection in Tibetan population in Qinghai; (2) the polymorphism of TNF- 伪 -308 gene may not be associated with the susceptibility to HBV infection in Tibetan population in Qinghai.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62

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