PD-1和PD-L1表达在慢性乙型肝炎病毒不同感染阶段患者的PBMCs表面表达
发布时间：2018-11-18 11:15
【摘要】：目的通过检测不同慢性乙型肝炎病毒(HBV)感染阶段的患者外周血单核细胞(PBMCs)表面程序性死亡受体-1(PD-1)及其配体(PD-L1)的表达,探讨其与血清HBV-DNA载量、ALT之间的相关性。方法收集不同临床类型慢性HBV感染者80例,其中HBV携带者20例(A组),1×ULN≤ALT2×ULN 20例(B组),2×ULN≤ALT5×ULN 20例(C组),ALT≥5×ULN 20例(D组),健康献血者20例(E组)。采用流式细胞术检测外周血单核细胞(PBMCs)表面PD-1及其配体PD-L1表达,以实时荧光定量PCR检测血清HBVDNA,同时检测血清谷丙转氨酶(ALT)。结果 A、B、C、D组PBMCs表面PD-1、PD-L1表达显著高于E组(P0.05);C、D组与A、B组间比较,PBMCs表面PD-1、PD-L1表达显著升高(P0.05),C组与D组比较PBMCs表面PD-1、PD-L1表达水平差异无统计学意义(P0.05);A组与B组比较,PBMCs表面PD-1、PD-L1表达水平差异无统计学意义(P0.05)。不同慢性HBV感染阶段患者PBMCs表面PD-1、PD-L1表达水平与血清HBV-DNA载量间无相关性(r=0.271,P0.05),但与ALT间有相关性(r=0.679,P0.05)。结论慢性HBV不同感染阶段患者PBMCs表面PD-1、PD-L1表达与机体免疫状态有关,肝脏炎症损伤是影响PD-1、PD-L1表达的重要因素,HBV-DNA载量不是主要影响原因。
[Abstract]:Objective to investigate the expression of programmed death receptor 1 (PD-1) and its ligand (PD-L1) on the surface of peripheral blood monocytes of patients with chronic hepatitis B virus (HBV) infection at different stages of (PBMCs) infection, and to investigate their association with serum HBV-DNA load. The correlation between ALT. Methods 80 patients with different clinical types of chronic HBV infection were collected, including 20 HBV carriers (group A), 1 脳 ULN 鈮，
本文编号：2339888
[Abstract]:Objective to investigate the expression of programmed death receptor 1 (PD-1) and its ligand (PD-L1) on the surface of peripheral blood monocytes of patients with chronic hepatitis B virus (HBV) infection at different stages of (PBMCs) infection, and to investigate their association with serum HBV-DNA load. The correlation between ALT. Methods 80 patients with different clinical types of chronic HBV infection were collected, including 20 HBV carriers (group A), 1 脳 ULN 鈮，
本文编号：2339888
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