中缅边境疟疾主动病例侦查范围与人群带虫者检测结果

发布时间：2018-11-25 18:30

【摘要】：目的探索我国中缅边境地区疟疾监测与响应模式中主动病例侦查范围,并了解当前该地区人群中无症状带虫者情况。方法 2014年7月在云南省盈江县选择存在疟疾本地感染病例的3个自然村作为调查现场。将这些本地感染病例作为线索病例,以其住所为圆心,分别以100 m、300 m、500 m和1 000 m为半径,侦查该范围内当地居住人群。收集每个被调查者耳垂血分别制作厚、薄血片各一张和滤纸血一份,采用显微镜观察和巢式PCR检测其疟原虫感染情况。结果共收集278例血样,显微镜观察发现3例无症状带虫者,人群中带虫率为1.1%(3/278);巢式PCR结果发现6例无症状带虫者,人群带虫率2.2%(6/278)。依据巢式PCR结果,不同侦查半径分析表明,300 m半径范围能够发现线索病例周围人群中全部无症状带虫者,其中在101~300 m范围内发现的占66.7%。结论在我国中缅边境地区人群中存在一定比例无症状带虫者,巢式PCR检测方法开展侦查半径为300 m的主动病例侦查能够有效地发现人群中无症状带虫者。
[Abstract]:Objective to explore the active case detection scope of malaria surveillance and response model in China-Myanmar border area and to find out the situation of asymptomatic carriers in this area. Methods in July 2014, three natural villages with malaria infection cases were selected as investigation site in Yingjiang County, Yunnan Province. These local infection cases were taken as clue cases and their residences were taken as the center of circle, with the radius of 100m ~ 300m ~ 500m and 1 ~ 000m respectively, to detect the local residents in this area. The blood samples of each ear lobe were collected to make a thick and thin blood piece of Zhang He filter paper blood. The infection of Plasmodium was detected by microscope and nested PCR. Results A total of 278 blood samples were collected, and 3 asymptomatic carriers were observed under microscope, the rate of infection was 1.1% (3 / 278) in the population, and 2.2% (6 / 278) in 6 asymptomatic carriers (6 / 278) by nested PCR. According to the results of nested PCR, the analysis of different detection radius showed that all asymptomatic carriers in the population around the clue case could be found within the radius of 300m, of which 66.7% were found within the range of 101 ~ 300m. Conclusion there is a certain proportion of asymptomatic carriers in the population in the border area of China and Myanmar. Nested PCR detection can effectively detect asymptomatic carriers in the population by detecting active cases with a detection radius of 300m.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织疟疾血吸虫病与丝虫病合作中心;山西医科大学公共卫生学院;
【基金】：亚洲消除疟疾合作网络(APMEN)资助项目(No.108-06)~~
【分类号】：R531.3

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