分枝杆菌噬菌体在抗结核研究中的作用
发布时间：2018-12-06 13:59
【摘要】：分子杆菌噬菌体是其宿主分枝杆菌的病毒,前者对于结核病控制及临床结核菌诊断有良好的应用前景。目前已有超过1000株分枝杆菌噬菌体进行了全基因组测序,结果显示分枝杆菌噬菌体基因组具有丰富的多样性和镶嵌结构,且都编码有噬菌体裂解酶。裂解酶是噬菌体在感染宿主末期表达的蛋白,其能水解细胞壁以释放子代噬菌体。除裂解酶外,分枝杆菌噬菌体还编码其它有抗结核活性的多肽及蛋白。分枝杆菌噬菌体在临床耐药结核菌的检测中也发挥了巨大作用。本文综述了来源于分枝杆菌噬菌体的多肽与蛋白的抗结核活性,并系统阐述了分枝杆菌噬菌体在临床结核菌耐药性检测中的应用,并展望了工程噬菌体在杀死耐药病原菌研究中的应用前景。
[Abstract]:Molecular bacteriophage is the host virus of Mycobacterium spp. The former has a good application prospect for tuberculosis control and clinical diagnosis of tuberculous bacilli. Up to now, more than 1000 strains of Mycobacterium phage have been sequenced. The results show that the phage genome of Mycobacterium has rich diversity and mosaic structure, and all of them encode phage lyase. Lyase is a protein expressed by phage at the end of infected host. It can hydrolyze cell wall to release offspring phage. Besides lyase, Mycobacterium phage also encodes other peptides and proteins with anti-tuberculosis activity. Mycobacterium bacteriophage also plays an important role in the detection of drug-resistant tuberculous bacteria. In this paper, the antituberculous activity of peptides and proteins from Mycobacterium phage was reviewed, and the application of Mycobacterium phage in the detection of drug resistance of Mycobacterium tuberculosis was systematically reviewed. The application prospect of engineering bacteriophage in killing resistant pathogenic bacteria was prospected.
【作者单位】： 河南大学基础医学院;西南大学生命科学学院现代生物医药研究所;
【基金】：2017年度河南省高等学校重点科研项目资助计划(No.17A310015)
【分类号】：R52
，
本文编号：2366137
[Abstract]:Molecular bacteriophage is the host virus of Mycobacterium spp. The former has a good application prospect for tuberculosis control and clinical diagnosis of tuberculous bacilli. Up to now, more than 1000 strains of Mycobacterium phage have been sequenced. The results show that the phage genome of Mycobacterium has rich diversity and mosaic structure, and all of them encode phage lyase. Lyase is a protein expressed by phage at the end of infected host. It can hydrolyze cell wall to release offspring phage. Besides lyase, Mycobacterium phage also encodes other peptides and proteins with anti-tuberculosis activity. Mycobacterium bacteriophage also plays an important role in the detection of drug-resistant tuberculous bacteria. In this paper, the antituberculous activity of peptides and proteins from Mycobacterium phage was reviewed, and the application of Mycobacterium phage in the detection of drug resistance of Mycobacterium tuberculosis was systematically reviewed. The application prospect of engineering bacteriophage in killing resistant pathogenic bacteria was prospected.
【作者单位】： 河南大学基础医学院;西南大学生命科学学院现代生物医药研究所;
【基金】：2017年度河南省高等学校重点科研项目资助计划(No.17A310015)
【分类号】：R52
，
本文编号：2366137
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