CD147在尖锐湿疣组织中的表达及其介导的发病机制

发布时间：2019-01-24 22:03

【摘要】：目的探讨CD147在尖锐湿疣组织中的表达及其在尖锐湿疣发病机制中的作用。方法收集30例尖锐湿疣(condyloma acuminata,CA)皮损组织作为CA组,另取30例男性包皮组织作为对照组,采用免疫组化染色检测组织CD147、PCNA、CD31表达,以CD31染色情况计算微血管密度(microvascular density,MVD),分析CA皮损组织CD147表达和PCNA、MVD的相关性。采用小干扰RNA(siRNA)技术抑制角质形成细胞HaCaT细胞中CD147的表达,按照处理方式分为空白对照组、CD147-siRNA组和CD147-NC组,MTT法检测细胞增殖能力,流式细胞术检测细胞凋亡率和周期分布,Real-time PCR检测CD147、VEGF、PCNA mRNA表达,Western blot检测VEGF、增殖细胞核抗原(PCNA)蛋白表达。结果 CA组皮损组织CD147表达阳性率为83.3%,PCNA表达阳性率为70.0%,对照组包皮组织分别为36.7%和40.0%,CA组CD147、PCNA表达阳性率明显高于对照组(均P0.05);CA组MVD值为(35.81±6.54)条/mm2,对照组为(12.74±4.75)条/mm2,CA组MVD值明显高于对照组(P0.01);相关性分析显示:CA组皮损组织CD147与PCNA表达呈正相关关系(r=0.753,P0.05),CD147表达与MVD值亦呈正相关关系(r=0.872,P0.05)。细胞实验中,与CD147-NC组比较,CD147-siRNA组处理48、72、96h后HaCaT细胞的增殖能力明显降低(均P0.05);CD147-siRNA组细胞凋亡率明显高于CD147-NC组(P0.01),CD147-siRNA组G2/M期所占比例明显低于CD147-NC组,S期所占比例明显高于CD147-NC组(均P0.01);CD147-siRNA组PCNA、VEGF的mRNA和蛋白表达明显低于CD147-NC组(均P0.01)。结论 CA皮损组织中CD147呈多数阳性表达,且CD147的表达与PCNA表达及MVD均呈正相关;下调CD147表达可以抑制角质形成细胞HaCaT的增殖,其作用机制可能与降低细胞周期蛋白和调控血管新生有关。
[Abstract]:Objective to investigate the expression of CD147 in condyloma acuminatum and its role in the pathogenesis of condyloma acuminatum. Methods 30 cases of condyloma acuminatum (condyloma acuminata,CA) lesions were collected as CA group and 30 cases of male prepuce tissues as control group. Immunohistochemical staining was used to detect the expression of CD147,PCNA,CD31. Microvessel density (microvascular density,MVD) was calculated by CD31 staining and the correlation between CD147 expression and PCNA,MVD in CA lesions was analyzed. Small interfering RNA (siRNA) technique was used to inhibit the expression of CD147 in HaCaT cells of keratinocytes. The cells were divided into three groups: blank control group, CD147-siRNA group and CD147-NC group. The proliferative ability of the cells was measured by MTT assay. Apoptosis rate and cell cycle distribution were detected by flow cytometry, CD147,VEGF,PCNA mRNA expression by Real-time PCR and (PCNA) protein expression by VEGF, proliferating cell nuclear antigen (VEGF,) by, Western blot. Results the positive rate of CD147 expression in lesions of CA group was 83.3%. The positive rate of CD147,PCNA expression in prepuce tissue of control group was 36.7% and 40.0%, respectively. The positive rate of CD147,PCNA expression in CA group was significantly higher than that in control group (P0.05). The MVD value of CA group (35.81 卤6.54) / mm2, control group (12.74 卤4.75) / mm2,CA group was significantly higher than that of control group (P0.01). Correlation analysis showed that there was a positive correlation between CD147 and PCNA expression in CA group (P 0.05), and a positive correlation between CD147 expression and MVD value (P 0.05). In cell experiment, compared with CD147-NC group, the proliferation ability of HaCaT cells in CD147-siRNA group decreased significantly (P0.05) after treatment for 48 ~ 72h for 96 h. The rate of apoptosis in CD147-siRNA group was significantly higher than that in CD147-NC group (P0.01). The percentage of G _ 2 / M phase in CD147-siRNA group was significantly lower than that in CD147-NC group, and the proportion of S phase was significantly higher than that in CD147-NC group (P0.01). The expression of mRNA and protein in PCNA,VEGF in CD147-siRNA group was significantly lower than that in CD147-NC group (P 0.01). Conclusion the expression of CD147 was mostly positive in the lesions of CA, and the expression of CD147 was positively correlated with the expression of PCNA and MVD. Down-regulation of CD147 expression can inhibit the proliferation of HaCaT in keratinocytes, and its mechanism may be related to the reduction of cyclin and the regulation of angiogenesis.
【作者单位】：广东省人民医院广东省医学科学院皮肤科;
【基金】：广东省医学科学技术研究基金资助项目(No.A2015148)
【分类号】：R752.53

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