田鼠巴贝虫隐性感染鼠再感染、免疫抑制或盲传后的虫密度消长规律研究

发布时间：2019-02-09 17:55

【摘要】：目的观察田鼠巴贝虫(Babesia microti)隐性感染期小鼠经再感染、免疫抑制或盲传健康小鼠后的外周血红细胞内虫体密度的消长规律。方法取1只感染种鼠的外周血(虫密度为20%),腹腔接种6周龄雌性BALB/c健康小鼠12只,100μl/只,用随机数字表法分为对照组、再感染组、免疫抑制组和盲传组,每组3只。4组感染小鼠连续28 d尾部采血,吉氏染色法观察田鼠巴贝虫形态变化,并计算红细胞染虫率,构建隐性感染小鼠模型。再感染组隐性感染小鼠再次腹腔接种相同剂量的田鼠巴贝虫感染种鼠血;免疫抑制组隐性感染小鼠腹腔注射地塞米松,0.5 mg/只,连续注射5 d;盲传组3只隐性感染小鼠取眼眶血,分别腹腔盲传接种健康雌性BALB/c小鼠各3只,100μl/只。3组小鼠继续尾部采血28 d,镜下计数感染红细胞,计算染虫率,并观察田鼠巴贝虫形态变化。结果对照组、再感染组、免疫抑制组和盲传组小鼠外周血均于感染后第3天查见田鼠巴贝虫体,第7天红细胞染虫率最高,分别为73.2%、78.0%、76.2%及79.0%,随后染虫率逐渐下降,至第28天外周血镜检阴性,进入隐性感染阶段。再感染组小鼠再次感染后28 d内,每天染虫率均为0。免疫抑制组小鼠在免疫抑制后第2天查见虫体,第12天染虫率再次达到高峰,为65.2%,随后逐渐下降,至第22天再次进入隐性感染期。盲传组新感染的9只小鼠在感染后第4天查见虫体,第9~10天染虫率达到高峰,为35.0%~39.0%,随后逐渐下降,至第28天小鼠进入隐性感染阶段。各组感染的田鼠巴贝虫形态变化基本一致,染虫初期以小环状体居多;染虫高峰期多见大环状体和长丝状体;有多虫寄生现象。结论田鼠巴贝虫隐性感染期小鼠有带虫免疫现象,并可作为传染源;经免疫抑制后虫体被激化,可出现与首次感染相同的虫体密度消长规律。
[Abstract]:Objective to observe the growth and decrease of the body density of erythrocytes in mice with (Babesia microti) recessive infection after reinfection, immunosuppression or blind transmission. Methods Peripheral blood (20% worm density) of one infected rat was inoculated intraperitoneally with 12 healthy female BALB/c mice (100 渭 l / mouse) at the age of 6 weeks. The mice were randomly divided into control group, reinfection group, immunosuppression group and blind transmission group. Three mice in each group were collected blood from the tail of infected mice for 28 days. The morphological changes of Babel beetle in voles were observed and the rate of erythrocyte infection was calculated to establish the model of recessive infection. Mice of recessive infection in reinfection group were intraperitoneally inoculated with the same dose of mice infected with Barb 茅 a vole, and mice in immunosuppressive group were injected dexamethasone intraperitoneally with 0.5 mg/ for 5 days. In the blind transmission group, orbital blood was taken from 3 recessive infected mice and inoculated intraperitoneally with 3 healthy female BALB/c mice (100 渭 l / mouse) respectively. The mice in the 3 groups continued to collect blood from the tail for 28 days. The infected red blood cells were counted under microscope and the rate of infection was calculated. The morphological changes of Barb 茅 e in voles were observed. Results in the control group, reinfection group, immunosuppressive group and blind transmission group, the mice were found in the peripheral blood on the 3rd day after infection. The erythrocyte infecting rate was the highest on the 7th day, 73.2% and 79.0%, respectively, and 78.2% and 79.0% in the control group, reinfection group, immunosuppression group and blind transmission group respectively. Then the infection rate decreased gradually, and the peripheral blood microscopic examination was negative on the 28th day, and entered the stage of recessive infection. Within 28 days after reinfection, the rate of infection was 0. 5%. On the second day after immunosuppression, the worm body was found in the immunosuppression group, and the rate of infection reached a peak of 65.2 on the 12th day, then decreased gradually, and entered the recessive infection stage again on the 22nd day. In the blind transmission group, 9 newly infected mice were detected on the 4th day after infection, and the infection rate reached a peak of 35.039.0 on the 9th day, and then decreased gradually, and the mice entered the stage of recessive infection on the 28th day. The morphological changes of the infected voles in each group were basically the same, most of them were small ring bodies at the initial stage of infection, and the large circular bodies and filamentous bodies were more common in the peak period of infection, and there were many parasites. Conclusion the mice in the recessive infection stage of Barb 茅 b's disease in voles are immune to the parasite and can be used as the source of infection, and the density of the parasite is the same as that of the first infection after the inhibition of immunity.
【作者单位】：复旦大学生命科学学院;中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合中心卫生部寄生虫病原与媒介生物学重点实验室;
【基金】：上海市卫计委青年基金项目(No.20154Y0155) 国家科技基础条件平台专项寄生虫病和热带病科技资源中心项目卫生公益行业科研专项(No.201202019)~~
【分类号】：R531

【相似文献】