登革热患者血清中细胞因子表达水平的临床分析
发布时间：2019-04-27 20:31
【摘要】：目的:研究不同细胞因子在登革热感染患者不同病程中的变化趋势,为临床诊治提供指导;分析细胞因子在登革热首次和再次感染中的差异,探讨其在登革热发病中的作用。方法:根据ELISA方法检测的IgG和IgM抗体水平,将收集的298例登革热患者血清分为首次和再次感染,用流式细胞术检测分析不同登革热感染患者发热后不同时期血清中的23种细胞因子水平,分析细胞因子水平在登革病毒感染患者血清中的特点以及临床意义。结果:登革热首次感染和再次感染患者血清中的细胞因子ENA-78、I-TAC、Mig、TARC、RANTES、MCP-1和IP-10水平在发热后不同阶段与健康对照比较均有显著差异(P0.05)。首次感染患者的IL-10、IFN-α和MCP-1水平在发热后1-4天(P0.05,P0.01,P0.001)、IFN-γ水平在发热后5-6天(P0.05,)、Mig水平在发热后5-6(P0.05)天和 7-8 天(P0.05)、TARC 水平在发热后 1-4 天(P0.01)和 5-6 天(P0.05)与再次感染比较有显著差异。结论:细胞因子在登革病毒感染后的发病过程起到重要作用,细胞因子水平的升高或者降低与疾病发展密切相关,而IL-10、IFN-α、MCP-1、Mig和TARC在登革热再次感染患者病情的发展中发挥着重要,可能是登革热再次感染患者病情加重的重要原因,这些细胞因子将在登革热疫苗研发方面有着重要的应用前景。
[Abstract]:Aim: to study the change trend of different cytokines in different course of dengue fever infection, to provide guidance for clinical diagnosis and treatment, to analyze the difference of cytokines in the first and second infection of dengue fever, and to explore the role of cytokines in the pathogenesis of dengue fever. Methods: according to the antibody levels of IgG and IgM detected by ELISA, the sera of 298 patients with dengue fever were divided into the first infection and the second infection. The levels of 23 cytokines in serum of patients with different dengue fever were detected by flow cytometry, and the characteristics and clinical significance of cytokine levels in serum of patients with dengue virus infection were analyzed. Results: the levels of cytokine ENA-78,I-TAC,Mig,TARC,RANTES,MCP-1 and IP-10 in serum of patients with first infection and reinfection of dengue fever were significantly different from those of healthy controls at different stages after fever (P0.05). The levels of IL-10,IFN- 伪 and MCP-1 in patients with first infection were 4 days after fever (P 0.05, P 0.01, P 0.001), and the levels of IFN- 纬 were 5 days after fever and 6 days after fever (P 0.05, P 0.05). The level of Mig was significantly different from that of re-infection at 5-6 (P0.05) and 7-8 (P0.05) days after fever (P0.05) and at 4 (P0.01) and 5-6 (P0.05) days after fever and re-infection, respectively. Conclusion: cytokines play an important role in the pathogenesis of dengue virus infection. The increase or decrease of cytokine level is closely related to the development of the disease, while IL-10,IFN- 伪 and MCP-1, are closely related to the development of the disease. Mig and TARC play an important role in the development of re-infection of dengue fever, which may be an important cause of exacerbation of patients with re-infection of dengue fever. These cytokines will have an important application prospect in the research and development of dengue vaccine.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R512.8;R446.6
本文编号：2467285
[Abstract]:Aim: to study the change trend of different cytokines in different course of dengue fever infection, to provide guidance for clinical diagnosis and treatment, to analyze the difference of cytokines in the first and second infection of dengue fever, and to explore the role of cytokines in the pathogenesis of dengue fever. Methods: according to the antibody levels of IgG and IgM detected by ELISA, the sera of 298 patients with dengue fever were divided into the first infection and the second infection. The levels of 23 cytokines in serum of patients with different dengue fever were detected by flow cytometry, and the characteristics and clinical significance of cytokine levels in serum of patients with dengue virus infection were analyzed. Results: the levels of cytokine ENA-78,I-TAC,Mig,TARC,RANTES,MCP-1 and IP-10 in serum of patients with first infection and reinfection of dengue fever were significantly different from those of healthy controls at different stages after fever (P0.05). The levels of IL-10,IFN- 伪 and MCP-1 in patients with first infection were 4 days after fever (P 0.05, P 0.01, P 0.001), and the levels of IFN- 纬 were 5 days after fever and 6 days after fever (P 0.05, P 0.05). The level of Mig was significantly different from that of re-infection at 5-6 (P0.05) and 7-8 (P0.05) days after fever (P0.05) and at 4 (P0.01) and 5-6 (P0.05) days after fever and re-infection, respectively. Conclusion: cytokines play an important role in the pathogenesis of dengue virus infection. The increase or decrease of cytokine level is closely related to the development of the disease, while IL-10,IFN- 伪 and MCP-1, are closely related to the development of the disease. Mig and TARC play an important role in the development of re-infection of dengue fever, which may be an important cause of exacerbation of patients with re-infection of dengue fever. These cytokines will have an important application prospect in the research and development of dengue vaccine.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R512.8;R446.6
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