凝血与补体级联反应信号通路对艾滋病湿热内蕴证发病的影响

发布时间：2019-04-29 07:23

【摘要】：目的明确艾滋病湿热内蕴证蛋白水平的表达差异,探索凝血与补体级联反应信号通路在中医证候实质中所主导的作用。方法 2014年8月根据整群随机抽样原则,纳入艾滋病患者48例(艾滋病肺脾气虚证25例为艾滋病肺脾气虚证组,艾滋病湿热内蕴证23例为艾滋病湿热内蕴证组),并以同地区人口资料相似的非HIV感染者16例为对照组,采集所有受试者血清进行i TRAQ技术研究,将差异蛋白进行通路注释。结果艾滋病湿热内蕴证组与艾滋病肺脾气虚证组相比,α1-抗胰蛋白酶、补体C4结合蛋白(C4bp)表达下调;艾滋病湿热内蕴证组与对照组相比,α1-抗胰蛋白酶、补体C4bp、α-2巨球蛋白、人结合丝氨酸凝集素蛋白酶2表达上调,补体C1r subcomponent、补体因子B及载脂蛋白B variant表达下调。这些差异蛋白均存在于凝血与补体级联反应信号通路。结论艾滋病湿热内蕴证的发病机制可能是通过凝血与补体级联反应信号通路实现的,该信号通路上的差异蛋白点可作为艾滋病临床治疗的潜在用药靶点。
[Abstract]:Objective to clarify the expression difference of protein level in dampness-heat syndrome of AIDS and to explore the role of cascade reaction signal pathway of coagulation and tonifying in the essence of syndrome of traditional Chinese medicine (TCM). Methods according to the principle of cluster random sampling in August 2014, 48 AIDS patients (25 cases of lung-qi deficiency syndrome and 23 cases of dampness-heat syndrome of AIDS) were included in the group of AIDS patients with lung-qi deficiency syndrome and 23 cases of dampness-heat internal accumulation syndrome of AIDS. 16 non-HIV infected subjects with similar population data were used as the control group. The serum samples of all subjects were collected for I-TRAQ study, and the differential proteins were annotated. Results the expression of 伪 _ 1-antitrypsin and complement C _ 4-binding protein (C4bp) were down-regulated in the dampness-heat syndrome group compared with the lung-spleen deficiency syndrome group. Compared with the control group, the expression of 伪 1-antitrypsin, complement C4bp, 伪-2 macroglobulin and human-binding serine agglutinin protease 2 were up-regulated, complement C1r-subcomponent, complement factor B and apolipoprotein B variant were down-regulated in the damp-heat syndrome group. These differential proteins exist in the cascade reaction signal pathway of clotting and tonifying. Conclusion the pathogenesis of dampness-heat syndrome of AIDS may be realized through the cascade reaction signal pathway of coagulation and tonifying reaction, and the differential protein spots in the signal pathway can be used as potential drug targets for the clinical treatment of AIDS. [WT5 "HZ] conclusion [WT5" BZ] [WT5 "BZ]
【作者单位】：河南中医药大学;河南省洛阳市第一中医院;
【基金】：国家十二五重大专项——HIV/AIDS中医证候转录组学研究(2012ZX10005001-005) 河南省高等学校重点科研项目——基于差异基因建立HIV/AIDS湿热内蕴证判别模型(15A360038) 河南中医学院博士科研基金——艾滋病中医发病机制“艾毒伤元”假说的物质基础的文献及实验研究(BSJJ2012-24)
【分类号】：R512.91

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