2015年佛山市首例本地登革热病例及蚊媒的病原分子溯源
发布时间：2019-05-21 14:50
【摘要】：目的对2015年佛山市报告的首例本地登革热疑似病例进行分子流行病学调查,确定病毒的基因型及感染来源。方法采集疑似患者血清标本、疫点白纹伊蚊成蚊及孑孓,采用Real-time PCR进行登革病毒基因检测及分型;用C6/36细胞分离登革病毒,采用套式RT-PCR扩增病毒E蛋白基因,进行序列测定和同源性比较分析。结果从患者血清和伊蚊研磨液中分别检测到1型登革病毒核酸,两者的病毒E蛋白基因核苷酸序列同源性为100%。从患者血清的C6/36细胞培养中观察到登革病毒特异的CPE。将分离株登革病毒命名为FS-D15001。通过对不同地区的登革毒株进行E蛋白基因核苷酸序列同源性比较,显示FS-D15001与2014年同一地区登革热患者分离毒株的同源性较高,为99.9%。结论经分子溯源证实2015年佛山市首例本地登革热病例由I型登革病毒引起,通过白纹伊蚊传播,该毒株在当地流行可能已持续数年。
[Abstract]:Objective to investigate the first suspected case of local dengue fever reported in Foshan in 2015 and to determine the genotype and source of infection. Methods the serum samples of suspected patients, Aedes albopictus adult mosquitoes and larvae were collected, and the dengue virus gene was detected and typed by Real-time PCR. Dengue virus was isolated from C _ 6 鈮，
本文编号：2482174
[Abstract]:Objective to investigate the first suspected case of local dengue fever reported in Foshan in 2015 and to determine the genotype and source of infection. Methods the serum samples of suspected patients, Aedes albopictus adult mosquitoes and larvae were collected, and the dengue virus gene was detected and typed by Real-time PCR. Dengue virus was isolated from C _ 6 鈮，
本文编号：2482174
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